The image on the right shows a typical µManager GUI. The details of the Groups and Presets may vary from system to system. Select the right Magnification Preset a magnification preset to match the objective lens and optovar (if applicable). Make sure the camera depth is set to its maximum the best quality (usually 12-bit). Click Live to get a live image. Click the Zoom tools to make the live image bigger or smaller. Set the exposure by typing a value in the field and change the binning if necessary. There may also be a gain control. Click the Auto button next to the live image contrast histogram to auto-automatically adjust the contrast of the image once, or you can tick Autostretch to have . Tick the Autostretch box to leave auto contrast on constantly (see below). Image Removed |