...
| Laser | Dichroic Mirror (reflection bands) | Emission Filter | Example Fluorophores |
|---|---|---|---|
| 405 | 400 - 410 | 430 - 485 | DAPI, Hoechst |
| 488 | 483 - 493 | 520 - 550 | GFP, FITC |
| 561 | 556 - 566 | 590 - 630 | RFP, mCherry |
| 640 | 635 - 645 | 680 - 740 | Cy5 |
The iSim has the following emission filters in Optospin Wheel 0
| Position | Laser | Emission band | Example Fluorophores |
|---|---|---|---|
| 1 | Any | 88000v2 Quad | Most |
| 2 | 405 | 410 - 510 | DAPI, Hoechst |
| 3 | 488 | 475 - 575 | GFP, FITC |
| 4 | 561 | 550 - 650 | tdTomato, TRITC |
| 5 | 561 | 555 - 705 | mCherry, Texas Red |
| 6 | 642 | 625 - 775 | Cy5 |
Camera Specifications
- Zyla 4.2 Plus specifications (Andor website). Note that the Zyla on GFP4 is a Camera Link model and not USB 3.0.
- Hamamatsu Orca-Flash4.0 v3 V3 specifications (Hamamamatsu Hamamatsu website)