Loading data

1. Click on the Analyze tab.
   
2. If you have just acquired a dataset it will already be listed in the Image Files: list in the top left of the UI..
   
3. To load a new dataset, click Load Data...

4. By default NimOS will localise molecules in real time during the acquisition, in which case the Result Files: list will already have a locb and nimb file in it.
   
   

   Note
   If Disable real-time localization was ticked prior to the acquisition then the Result Files list will be empty.

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Viewing images

There are two views of the data: The small image on the left is a thumbnail of the entire field of view; the larger image on the right can be zoomed on and examined up close. When you zoom on the image an ROI appears in the thumbnail, showing you the area you've zoomed on. You can zoom on the image by either using the track-wheel of a mouse (if you have one), or pinching two fingers together or apart on the laptop touchpad. You can reset the zoom by right-clicking and selecting Reset zoom.

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1. In the Viewing Options area in the lower right of the user interface you can tick or untick the Raw or Localizations columns to display the raw image data and/or localisation image (or both superimposed on one another).
   
2. Double-clicking the block of colour in the Colour column opens a dialogue box that will allow you to change the colour and rendering for each channel..
   
3. If the Raw column is ticked you can scroll through the Frame index of the acquisition sequence.
   

Calculate Drift Correction

There is a Calculate Drift Correction button amongst the Tools in the bottom left of the UI.

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