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The iSim has the following emission filters in Optospin Wheel 0
| Position | Laser | Emission band | Example Fluorophores |
|---|---|---|---|
| 1 | Any | 88000v2 Quad | Most |
| 2 | 405 | 410 - 510 | DAPI, Hoechst |
| 3 | 488 | 475 - 575 | GFP, FITC |
| 4 | 561 | 550 - 650 | tdTomato, TRITC |
| 5 | 561 | 555 - 705 | mCherry, Texas Red |
| 6 | 642 | 625 - 775 | Cy5 |
Camera Specifications
- Zyla 4.2 Plus specifications (Andor website). Note that the Zyla on GFP4 is a Camera Link model and not USB 3.0.
- Hamamatsu Orca-Flash4.0 V3 specifications (Hamamatsu website)
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