The image on the right shows a typical µManager GUI. The details of the Groups and Presets may vary from system to system. Select a magnification preset to match the objective lens and optovar (if applicable). Make sure the camera depth is set to the best quality (usually 12-bit). Click Live to get a live image. Click the Zoom tools to make the live image bigger or smaller. Set the exposure by typing a value in the field and change the binning if necessary. There may also be a gain control. Click the Auto button next to the live image histogram to automatically adjust the contrast of the image once. Tick the Autostretch box to leave auto contrast on constantly. |