...
Most
...
microscopes
...
are
...
widefield
...
microscopes.
...
In
...
widefield
...
microscopy
...
the
...
entire
...
field
...
is
...
simultaneously
...
illuminated
...
throughout
...
its
...
full
...
width
...
and
...
depth
...
with
...
the
...
...
...
...
of
...
the
...
objective
...
lens
...
determining
...
how
...
much
...
of
...
the
...
specimen
...
appears
...
in
...
focus.
...
Imaging
...
and
...
processing
...
of
...
single
...
planes
...
can
...
be
...
quick
...
and
...
easy
...
because
...
an
...
entire
...
field
...
can
...
be
...
captured
...
at
...
once
...
in
...
a
...
single
...
camera
...
image.
...
Widefield
...
is
...
most
...
suitable
...
for
...
2D
...
imaging
...
of
...
relatively
...
thin
...
specimens
...
because
...
all
...
the
...
depth
...
information
...
is
...
in
...
a
...
single
...
image
...
so
...
only
...
a
...
handful
...
of
...
images
...
needs
...
to
...
be
...
processed.
...
It
...
is
...
generally
...
not
...
as
...
useful
...
for
...
acquiring
...
3D
...
data
...
over
...
a
...
greater
...
depth
...
because
...
light
...
from
...
out
...
of
...
the
...
plane
...
of
...
focus
...
can
...
be
...
seen
...
as
...
blur
...
in
...
the
...
focal
...
plane.
...
The LMCB widefield microscopes are listed below:
Upright Microscopes
- Axio Imager (fluorescence, phase contrast, greyscale camera for sensitivity)
- Axioplan (fluorescence, differential interference contrast, greyscale camera for sensitivity)
Inverted Microscopes
- Leica 2 (fluorescence, phase contrast, colour camera for histological stains)