Basic procedure for setting up a

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timelapse experiment using GFP3

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Make sure the Oko-lab environmental chamber is set to the correct conditions for your experiment. In no particular order turn on the computer and the red button on the expansion strip labelled 'GFP3 Devices' behind the microscope. The red button turns on the following devices: microscope stand, motorised stage, light sources and camera. Login to the User account; there’s no password. Double-click the NIS-Elements icon on the desktop and enter your user credentials.

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Setting up the length of the timelapse and frequency of acquisition

Click the Timing… button to check how long a single loop will take; this will determine the minimum Interval between time points. Click the Time tab and select at least one tick box at the start of a row. Specify the desired Interval and a Duration or number of Loops. Ticking a box in the row below will create another time Phase, so for instance you can have a fast acquisition for a short period followed by a slow acquisition over a longer period. Click the ‘Close Active Shutter when Idle’ box to reduce photobleaching.

Click ‘1 1 time loop’ to loop to test the settings or ‘Run Now’ Run Now to begin the timelapse.