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After training, users are given an account on the Agendo booking system that will allow them to reserve time on the microscopes or analysis computers. Please see the Agendo Quick Start GuideIntroduction for information on how to start using the booking system. New users will also be added to the lmcb-lm-users@ucl.ac.uk mailing list. If you haven't been added to this list then you can subscribe at the following address: http://www.mailinglists.ucl.ac.uk/mailman/listinfo/lmcb-lm-users.

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• All microscopes MUST be booked before use; even if this means booking immediately before use. The Agendo system can be accessed from the confocal microscope computers, so a reservation can be made from the microscope system before the start of a session if necessary.
• The system will not allow you to reserve an appointment if the start time has already passed. If you cannot reserve time because of this, reserve whatever time you subsequently require for the current session. You may also send an email to lmcb-lm-help@ucl.ac.uk to ask a member of LM staff to book time in the past
• If you have reserved time on a microscope, but you know you will not be able use it, you MUST delete the reservation from the booking system before the start of the reserved time.
• If you cancel your slot less than 2 hours before it is due to begin you MUST send an alert to the Agendo or lmcb-lm-users@ucl.ac.uk mailing lists (see section on alerting users below). This is to prevent the machine being left on by previous users who still think you are going to use it, and to alert potential users to the free slot.
• Whenever you finish or cancel your session you must MUST find out if anyone has reserved a slot after you. You may have received an email with this information, but you must still check the booking system. This must be done at the end of the time you reserved, if  if possible.
• If more than three hours will elapse between the end of your reserved time and the beginning of the next reserved slot you must you are the last user you MUST shut the machine down, even if it is early in the day. This should be done after you use your slot or , if you do not want to use the slot, immediately after you cancel ityour slot. Note that there are some exceptions to this rule. The Elyra should always be left on and the analysis computers should be left on but logged out.
• If you want to cancel your reservation after the start of your time slot, or if you finish more than an hour early, you should MUST send an email to lmcb-lm-users@ucl.ac.uk the mailing list to alert others. You are responsible for shutting the machine down if there are no users booked after you for three hours. Note that the booking system does not allow you to delete slots that have already begun.

The above rules are designed to preserve the life of the confocal systems ; particularly the lasersand to ensure everybody has fair access to the machines. Failure to observe the rules them will initially result in a warning email. If a user continues to leave the machines on, the user will be sin binned and , after which booking privileges will be removed; initially for one week but increasing to two weeks, three weeks etc. if the problem continues. Warnings and sin binnings other penalties will elapse after six months if there have been no more infringements.

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• Send an email to lmcb-lm-users@ucl.ac.uk. This will alert everybody on the mailing list
• Use the built-in mailing list in the Agendo main menu, which can be used to alert users of particular machines (see Agendo Quick StartIntroduction)

In addition, if you want to be alerted when a single slot on a microscope or other resource becomes free you can add yourself to the Waiting List for that slot in Agendo. It is also possible to receive alerts when all slots are deleted or modified for a particular resource in My resource permissions.

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The following rules currently apply to the following confocal and super-resolution microscopes: SPE Radiance, SPE, SPE2, SPE3, SP5, Olympus FV1200 Multiphoton, Elyra, STED.

• Prime time: 1000 0900 to 1800 1700 Monday to Friday
• No restrictions on booking outside prime time
• Maximum number of hours per user per day in prime time: 4
• Maximum number of hours per user per week in prime time: 12
• You may only book 2 weeks in advance

Radiance - Users can book up to 24 slots per two-week period. The number of slots that can be booked per user per day is currently unrestricted.Vox - The Vox has extensive Vox and 3i spinning disc microscopes have additional rules because of the particular requirements of live imaging and time-lapse. See this PDF

GFP timeTime-lapse microscopes 1 & 2 - The GFP time-lapse microscopes have extensive GFP1, GFP2 and GFP3 have additional rules because of the particular requirements of live imaging and time-lapse. See this PDF

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Nikon N-STORM

Each user is allowed to book the microscopes for up to 4 hours per day between the core hours (9 am – 5pm). Outside the core hours and at the weekend users can book the microscopes for as long as they wish. Per week each user is allowed to book the microscopes for a total of 16 h during the core hours, e.g. 4 x 4 h. Slots can be booked up to two weeks in advance.