Interference Reflection Microscopy (IRM) uses reflected polarised light is normally used to study cellular interfaces contact sites on a glass surface without using fluorescent labels. Confocal microscope lasers are polarised, which makes them ideal for IRM. You can acquire fluorescence images at the same time as reflection images but with the White Light Laser (WLL) on the LMCB SP8 a notch filter will be needed to stop reflected light being detected in the fluorescence channels.
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