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The microscope is a fully motorised DMI 6000 CS inverted model with AFC auto focus, a Märzhäuser motorised scanning stage and a high speed Z Galvo focus in addition to the stand's internal focus motor. The microscope is full enclosed by a blacked-out chamber and is equipped with a Life Imaging Services Cube chamber heater and Brick CO2 gas mixer. Differential Interference Contrast (DIC) is available for the 10x, 20x and 40x lenses. Fluorescence can be viewed using the EL6000 metal halide lamp and the filter sets below.

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The confocal/STED scanning system has three HyD high-sensitivity hybrid detectors and two standard photo-multiplier (PMT) detectors for fluorescence; and one . It also has a transmitted light detector (TLD) for bright-field or DIC imaging.

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DETECTORS

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3x HyD, high sensitivity, 3 operating modes (standard, counting, BrightR) + time-gating

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3x PMT, one of which for transmitted light imaging

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INCUBATION: The microscope is fully enclosed and equipped with The Cube chamber heater and The Brick CO2 gas mixer.

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SOFTWARE 

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Acquistion

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LAS X

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Deconvolution

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Huygens Professional Version 15.10.1p2

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ADDITIONAL INFORMATION:

The HyD detectors are very sensitive and have a time-gating function that is critical for gated STED, so they should always be used for STED imaging.

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Additional Information

• resonant scanner (8000 Hz) for fast imaging
• anti-vibration optical table
• fully enclosed
• autofocus
• motorized XY stage
• Z-galvo