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| Light source | Type | Wavelengths [nm] |
| Semiconductor Diode | Laser | 405, 440, 473 |
| Semiconductor Diode | Laser | 559 |
| Semiconductor Diode | Laser | 635 |
| Multi-line Argon ion | Laser | 458, 488. 515 |
| Ablation laser | Laser (pulsed or cw) | 405 |
| U-HGLGPS | Mercury metal halide bulb | ~ 300 - 700 (peaks at 365, 405, 436, 546, 579) |
A
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OBJECTIVE LENSES
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| Magnification | Immersion | MediumType | Numerical ApertureNA | Coverslip correction [mm] | Working DistanceWD [mm] | UPLSAPO10X2|||
|---|---|---|---|---|---|---|---|---|
| 10x | air | UPLSAPO10X2 | 0.4 | N/A0.17 | 3.1UPLSAPO20X | |||
| 20x | air | UPLSAPO20X | 0.75 | N/A0.17 | 0.6 | |||
| UPLSAPO30XS40x | 30xoilsilicone oil | UPLFN40X0 | 1.05correction ring for multiple thicknesses and temeratures3 | 0.17 | 0.82 | |||
| UPLFLN40XO60x | 40xoiloil | PLAPON60X0SC2 | 1.3N/A4 | 0.17 | 0.2UPLSAPO60XS12 | |||
| 60x | silicone oil | UPLSAPO60XS | 1.3correction ring for multiple thicknesses and temeratures | 0.15 - 0.19 | 0.3 | PLAPON60XOSC | 60x | oil |
| 30x | silicone oil | UPLSAPO30XS | 1.05 | 0.13 - 0.19 | 0.8 | |||
| 60x* | water | UPLSAPO60XW | 1. | 4N/A2 | 0.13 - 0.21 | 0. | 12
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| 28 | |||||
NA = Numerical Aperture, WD = Working Distance | |||||
There is also a 60x UPLSAPO60XW water lens with NA 1.2, WD 0.28 and a correction collar for coverslip thicknesses 0.13 to 0.21 mm. It is kept in a drawer because there isn't a free position in the nose piece. If you need to use this leans then please ask a member of the Light Microscopy Facility staff because the microscope software settings must be edited when an objective lens changes.
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FLUORESCENCE FILTER SETS: Filter sets for imaging a wide range of standard visible and far-red fluorophores are available.
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- 3 channel PMT (Hamamatsu R7862)
- External transmitted light PMT (Hamamatsu R7400)
- Ultra-high sensitivity detector GaAsP with onboard Peltier cooling system (2 channel)
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INCUBATION
TOKAI HIT CO2 : TokaiHit CO2 stage top incubator including lens heater heater
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SOFTWARE
: FV10-ASW 4.2
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ADDITIONAL INFORMATION
- anti-vibration optical table
- motorized Z-drift compensator (ZDC)
- motorized XY stage
- Silver coated galvo scanner
- SIM scanner for simultaneous photostimulation and confocal image acquisition