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*Note that the transmitted light technique Differential Interference Contrast (DIC or Nomarski Contrast) is available on several of the confocal microscopes but is taught in advanced sessions rather than introductory training
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- How to calibrate the motorised stage (if necessary)
- How to set up a multi-well plate specimen for imaging at 37 degrees C in a CO2 atmosphere
- How to use the other available specimen inserts
- How to set up single and multi-point acquisitions with or without auto-focus
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- Differential Interference Contrast (DIC aka. Nomarski Contrast) on confocal microscopes
- Imaging of samples in non-standard or home-built specimen holders
- Live imaging of any kind
- Photo-bleaching, photo-activation and photo-conversion techniques (e.g. FRAP, FLIP, etc)
- Fluorescence Resonance Energy Transfer (FRET)
- Techniques requiring the use of a motorised stage (e.g. multi-position time-lapse, tiling/stitching)
- Multiphoton imaging and photo-ablation
- Fluorescence Correlation Spectroscopy (FCS) or Fluorescence Cross-correlation Spectroscopy (FCCS)
Advanced training will consist of a session where you will operate the microscope with one of your own specimens under the supervision of a member of the light microscopy facility. It is likely that some specimens (live specimens in particular) will require specific stage adapters and environmental conditions so please give details of the specimen to the staff member who will be supervising you so that the necessary arrangements can be made. It is also important that you check with the light microscopy facility to find out whether your particular procedure or specimen can be accommodated using the current equipment in the facility.
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