Agendo scheduling system rules

Limitations on booking within the scheduling system are listed below. Prime time limitations apply between 1000 and 1800 hours.

• Prime time: 1000 to 1800 Monday to Friday
• No restrictions on booking outside prime time
• Maximum number of hours per user per day in prime time: 5
• Maximum number of hours per user per week in prime time: 12
• You may only book 2 weeks in advance

Booking rules

In addition to the General Rules, the following specific booking rules apply.

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The rules are designed to preserve the life of the confocal system and to ensure everybody has fair access to the machines. Failure to observe them will initially result in a warning email, after which booking privileges will be removed; initially for one week but increasing to two weeks, three weeks etc. if the problem continues. Warnings and other penalties will elapse after six months if there have been no more infringements.

Usage rules

In addition the usage rules below must be followed.

• Always remove latex or nitrile gloves before touching the keyboard, mouse, microscope focus and buttons to prevent contamination with biological material and immersion oil
• Always keep immersion oil bottles in the petri dishes provided. This is to stop oil getting everywhere. Do not put anything else in the dishes
• Always keep stage adaptors, inserts and relevant immersion oils inside the chamber so they stay at the same temperature as the microscope
• If you are carrying out confocal imaging of specimens overnight or for longer you MUST turn off the Exfo X-Cite lamp on the shelf above the Vox and the HXP 120V lamp beneath the 3i table. They aren't needed for confocal imaging and leaving them on shortens the life of the bulb unnecessarily. Note that the 3i can also be used for wide-field time-lapse, in which case the lamp will be needed
• You must NEVER leave the microscope stand with the objective lens nose piece in the Escape (Nikon) or Load (Zeiss) position. This is because the next user will not be able to focus with the Escape or Load button pressed. Also, if they work out what has happened and press the refocus button the objective lens may go straight through their sample
• You must wipe all the lenses you used after you’ve finished your session. Only use the Whatman 105 lens cleaning tissue to wipe lenses
• Always clean up check carefully for any spilt medium on or around the microscope stage, nose piece and inside the chamber. Clean up any spills. The salts in media react with the metal of the microscope and can cause serious damage
• You MUST dispose of your specimens according to the LMCB Code of Practice for Laboratory Workers. Gloves and tissues should be discarded in the yellow clinical waste bag. Sharps and small biological specimens (e.g. Lab-Teks and 35 mm dishes) may be placed in the sharps bin provided, but any larger biological specimens must be taken away and treated overnight with a suitable disinfectant before disposal.

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