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Unless otherwise stated the microscope objective lenses require specimens to be mounted on a #1.5 glass coverslip (170 microns) or in a chamber/dish with a coverslip bottom of the same thickness.

Widefield Microscopes

Use a widefield microscope if your specimen is thin and relatively free of out-of focus blur; or you don't need to optically section it for processing, analysis and visualisation in 3D.

Microscope Name

Upright or Inverted?

Objective Lenses

Fluorescence Colours

Key Features

Techniques

Axioplan

Upright
for slides
Up to 100x

Blue, Green, Red, Far-Red

Zeiss Axioplan 2 microscope

with

. QImaging QClick camera

for high

. Fluorescence. DIC.

High sensitivity and high resolution fluorescence microscopy and differential interference contrast (DIC) of fixed specimens on slides

Axio Imager

Upright

for slides

Up to 100x

Blue, Green, Red, Far-Red

(

. Cyan and Yellow available on request

)

Zeiss Axio Imager A1 microscope

with

. QImaging Retiga EXi

for high

camera. Fluorescence. Phase contrast

High sensitivity and high resolution fluorescence microscopy and phase contrast of fixed specimens on slides

Axioskop 3

Upright

for slidesUp to 100x

Blue, Green, Red

Zeiss Axioskop microscope

with

. QImaging QICAM colour camera

for moderate

. Fluorescence. Phase contrast. Motorised stage

Moderate to high resolution fluorescence microscopy and imaging of histologically or histochemically stained specimens on slides. Motorised stage supports tiling and stitching of specimens.

Leica

Inverted

for TC dishesLong distance air lenses up to 40x

Blue, Green, Red

Leica DMIRB microscope

with

. Hamamatsu Orca NRB camera. Fluorescence. Phase contrast. Long distance lenses suitable for use with normal TC dishes and plates

Good for moderate resolution fluorescence microscopy and phase contrast of fixed/live specimens on slides, or in dishes and plates with glass or plastic bottoms.

Leica 2

Inverted

for TC dishesLong distance air lenses up to 40x

Blue, Cyan, Green, Red

Leica DMIRB microscope

with

. Leica DFC7000T colour camera. Fluorescence. Phase contrast. Leica Modulation Contrast (LMC). Long distance lenses suitable for use with normal TC dishes and plates

Moderate to high resolution fluorescence microscopy and colour imaging of histologically or histochemically stained specimens on slides, or in dishes and plates with glass or plastic bottoms. Leica Modulation Contrast (LMC) lenses for brightfield contrast imaging that is less sensitive to the meniscus in well plates. Cyan filter cube for CFP.

Confocal Microscopes

Use a confocal microscope if your specimen in thick, has significant out-of-focus blur, or if you need to process, analyse or visualise it in 3D.

Microscope Name

Upright or Inverted?

Objective Lenses

Laser Lines

FeaturesLive Imaging

Techniques

Radiance

SPE

Upright

for slides

Up to 40x oil (water dipping lenses available)

458

405, 488,

514

532,

543, 635Bio-Rad Microscience Radiance 2100 with Nikon 800 stand and three high-sensitivity GaAsP detectors for

640

Leica TCS SPE with DM2500 stand. 1x PMT detector. DIC imaging.Perfusion chamber and dipping lenses on request.

3D imaging of fixed specimens on slides

. DIC

. Live imaging

of specimens in gasket sealed chambers or open dishes possible using a heated stage adapter and lens heater. Fluorescence recovery after photobleaching (FRAP).

SPE

Upright for slides

Up to 63x oil (water dipping lenses available)

possible but not fast. Orthogonal (XZ) scans possible with the Z Galvo.

SPE2

Upright

405, 488, 532, 640

Leica TCS SPE with DM2500 stand
for 3D imaging of fixed specimens on slides. Live imaging of specimens in dishes possible with water dipping lenses using Oko-lab stage adapter or Warner Instruments perfusion chamber. DIC (not dipping lenses). Spectral imaging.

SPE

Upright for slides

Up to 63x oil (water dipping lenses available)

405, 488, 532, 640

Leica TCS SPE with DM2500 stand for
. 1x PMT detector. DIC imaging.Oko-Lab stage adapter and dipping lenses on request.

3D imaging of fixed specimens on slides. Live imaging

of specimens in dishes possible with water dipping lenses using Oko-lab stage adapter or Warner Instruments perfusion chamber. DIC (not dipping lenses). Spectral imaging

possible but not fast. Orthogonal (XZ) scans possible with the Z Galvo.

SPE3

Inverted

10x air to 63x oil

405, 488, 561, 640

Leica TCS SPE
version 2
with DMI4000 stand. 1x PMT detector. DIC imaging. Motorised stage. Matrix screener module.No incubation equipment is available for this microscope.

3D imaging of fixed specimens

on

in slides and

in

dishes. Fluorescence and DIC

. Spectral Imaging. Motorised stage for multi

on grids for CLEM. Multi-position

acquisition

imaging and tiling. Matrix Screener

software

for high content screening applications.

SP5

Inverted

10x air to 63x oil (63x glycerol available)

405, 457, 476, 488, 495, 561, 594, 633

Leica TCS SP5 with DMI6000B stand. 3x Hybrid and 1x PMT detectors. DIC. Motorised stage. FRAP and FRET wizards. Live Data Mode and Matrix Screener.Pe-Con stage-top incubator for 35 mm dishes.

4D (3D+T) imaging of

specimens on slides and live imaging of specimens in 35 mm dishes and Lab-Tek chambered coverslips using Pe-Con incubation equipment. Spectral imaging. DIC. Motorised stage for multi-position acquisition and tiling. FRAP wizard. Förster Resonance Energy Transfer with FRET wizard. Experiment design using Live Data Mode. Matrix Screener for high content screening.Spinning Disc Confocal Microscopes

live and fixed specimens. CLEM. Multi-position acquisition and tiling. FRAP and FRET Acceptor Photobleaching or Sensitised Emission. Complex experiment design.

Olympus FV1200Inverted

405, 458, 488, 515, 559, 635. Sim Scanner: 405 (pulsed for ablation), 440

Olympus FV1200 with IX83 stand. 3x PMT, 2x GaAsP detectors. Second scanner for simultaneous imaging and ablation. Motorised stage. Silicon lenses. Time Controller module.Tokai-Hit stage-top incubator with adaptors for dishes and chambered coverslips.

4D (3D+T) imaging of live and fixed specimens. High sensitivity imaging of green and red dyes with GaAsP detectors. Ablation of actin cortex. CLEM. Multi-position acquisition and tiling. Imaging thicker specimens. Complex experiment design.

MultiphotonInverted405, 458, 488, 514, 561, 633. Tune-able Ti-Sapphire and OPOZeiss LSM 880 NLO with Axio Observer Z1 stand. 32 channel GaAsP, 2x PMT, 2x NDD and BiG2. Airyscan. Motorised stage. FCS/FCCS. Experiment Designer.Pe-Con cage incubator with CO2 chamber for 35 mm dishes4D (3D+T) imaging of live and fixed specimens. Multiphoton imaging and ablation. Fast spectral imaging and un-mixing. Super-resolution. Multi-position acquisition. Complex experiment design.

Radiance

Upright

458, 488, 514, 543, 635

Bio-Rad radiance 2100 with Nikon E800 stand. 3x GaAsP detectors. Transmitted light contrast by DIC. Basic FRAP capability.Tokai-Hit stage heater and Bioptechs objective lens heater available on request.

3D imaging of fixed specimens on slides.

Spinning Disc Confocal Microscopes

Use spinning disc microscopes for high-speed live confocal imaging of samples.

Microscope Name

Upright or Inverted?

Objective Lenses

Laser Lines

Key FeaturesLive Imaging

Techniques

Vox

Inverted

10x air to 100x oil (60x water immersion available)

405, 488, 561, 640

Perkin Elmer Ultraview Vox with Yokogawa CSU-X1 DSU and Nikon TiE stand. Hamamatsu C9100-13 EMCCD camera. Photokinesis Unit for FRAP. Motorised stage. Piezo focus.

Imaging of live specimens in 35 mm dishes, Lab-Tek chambered coverslips and Ibidi uSlides using Solent Scientific cage type incubation chamber.

Sensitive and gentle high-speed live confocal imaging. This machine has a smaller field of view than the 3i but the image acquisition speed is higher. DIC. Multi-point and stitching. Good for FRAP. CLEM.

3iInverted
10x air to 100x oil (40x water immersion available)
405, 440, 488, 514, 561, 640
Imaging of live specimens in

Yokogawa CSU-W1 spinning disc and Zeiss Axio Observer Z1 stand. Photometrics Prime 95b and Andor iXon 888 Life EMCCD cameras. Vector for FRAP. Motorised stage. High speed piezo focus

Oko-Lab cage incubator with CO2 chamber and adaptors for 35 mm dishes,
Lab-Tek
chambered coverslips and
Ibidi uSlides using Oko-Lab cage type incubation chamber. DIC
multi-well plates.Sensitive and gentle high-speed live confocal imaging. This machine has a larger field of view than the Vox but the image acquisition speed is not quite as high. Multi-point and stitching. FRAP.

Time-lapse inverted microscope systems

Microscope

Objective Lenses
Upright or Inverted?

Fluorescence Colours

CameraGreyscale high sensitivity CCD

Key Features

Live Imaging

Techniques

GFP1

10x to 40x air and LWD (oil lenses available)

Green and Red

Inverted

Green, Red, Far-Red

Zeiss Axio Observer Z1 stand with Hamamatsu Orca Flash4.0 v2 sCMOS camera. Fully automated with motorised stage. Fluorescence and phase contrast.

Solent Scientific chamber with adaptor for multi-well plate. Other adaptors available on request.

Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence.

Stage adapters support Nunc and Mat-Tek multi-well plates and 35 mm Mat-Tek dishes. If you need to use another vessel or adapter you must contact Andrew Vaughan

GFP2

10x to 40x air and LWD (oil lenses available)

Blue, Green and Red

Greyscale high sensitivity CCD

GFP2

Inverted

Cyan, Green, Red, Far-Red

Zeiss Axiovert 200M stand with Hamamatsu Orca ER CCD camera. Fully automated with motorised stage. Fluorescence and phase contrast. Long distance lenses.

Solent Scientific chamber with adaptor for multi-well plate. Other adaptors available on request.

Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence. Long distance lenses suitable for use with normal TC dishes and plates

GFP3

Inverted

Blue, Green and Red. Cyan and Yellow available on request.

Nikon TiE stand. DS-Qi2 sCMOS camera with wide field of view. Fully automated with motorised stage. Perfect Focus System. Fluorescence and phase contrast. Long distance lenses.

Oko-Lab cage incubator with adaptors for multi-well plates, 35 mm dishes and chambered coverslips.

Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence

. Stage adapters support Nunc and Mat-Tek multi-well plates and 35 mm Mat-Tek dishes. If you need to use another vessel or adapter you must contact Andrew Vaughan

N-STORM (coming soon)

10x air to 100x oil TIRF lens

Blue, Green, Red and Far-Red

Greyscale ultra-high sensitivity EMCCD

Optimally configured for high resolution and high sensitivity fluorescence microscopy of living specimens in XYZT for deconvolution, TIRF and super-resolution using N-STORM and dSTORM

. Long distance lenses suitable for use with normal TC dishes and plates. Perfect Focus System ensures that the sample remains in focus over long periods.


Super-resolution Microscopes

MicroscopeUpright or InvertedLaser LinesKey FeaturesLive ImagingTechniques
ElyraInverted405, 488, 561, 642Zeiss Elyra PS.1 with Axio Observer Z1 stand. Andor iXon Du-897 EMCCD for PALM. PCO Edge sCMOS for SIM. Motorised stage and piezo focus. Single-band filter turret and optional dual-band filter turret for high speed dual channel imaging. 3D SIM and 3D PALM capabilities.Tokai-Hit stage-top incubator with adaptors suitable for 35 mm dishes and chambered coverslips.Structured Illumination Microscopy, PALM and dSTORM, Total Internal Reflection (TIRF) microscopy
GFP4Inverted405, 488, 561, 642Visitech vt-iSIM and Nikon TiE stand. Hamamatsu Orca Flash4.0 v3 camera for vt-iSIM. Andor Zyla for wide-field imaging. Motorised stage and piezo focus.Oko-Lab cage incubator with adaptors for multi-well plates, 35 mm dishes and chambered coverslips.Instant SIM that gives a live structured illumination image without processing. Further resolution possible with deconvolution. Wide-field.
N-STORMInverted405, 488, 561, 647Nikon N-STORM and Ti stand. Andor iXon Du-897 EMCCD camera for STORM and dSTORM. Astigmatic lens for 3D STORM. Zyla sCMOS camera for wide-field imaging.Solent Scientific cage incubator with adaptors for multi-well plates and 35 mm dishes.Stochastic Optical Reconstruction Microscopy (STORM) and direct STORM. TIRF microscopy. Wide-field time-lapse.
STEDInverted405, WLLLeica TCS gSTED 3X with DMI6000B stand. 3x Hybrid and 2x PMT detectors. Motorised stage. High speed Z Galvo focus. 532, 660 and 775 nm depletion lasers for multi-colour STED. 3D STED capability.Life Imaging Services Box cage incubator system with adaptors for 35 mm dishes and chambered coverslipsMulti-colour STED and confocal microscopy. 3D STED. Tiling of large specimens.