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Use a widefield microscope if your specimen is thin and relatively free of out-of focus blur; or you don't need to optically section it for processing, analysis and visualisation in 3D.

Microscope Name

Upright or Inverted?

Fluorescence Colours

Key Features

Techniques

Axioplan

Upright

Blue

, Green

, Red

, Far-Red

  • Fluorescence
  • DIC

Zeiss Axioplan 2 microscope

with

. QImaging QClick camera

for high

. Fluorescence. DIC.

High sensitivity and high resolution fluorescence microscopy and differential interference contrast (DIC) of fixed specimens on slides

Axio Imager

Upright

Blue

, Green

, Red

, Far-Red

. Cyan and Yellow available on request

Fluorescence

  • Phase contrast

    • Zeiss Axio Imager A1 microscope

    with

    . QImaging Retiga EXi

    for high

    camera. Fluorescence. Phase contrast

    High sensitivity and high resolution fluorescence microscopy and phase contrast of fixed specimens on slides

    Axioskop 3

    Upright

    Blue

    , Green

  • Red
  • Fluorescence
  • Phase contrast
  • Colour camera
    • Motorised stage

    , Red

    Zeiss Axioskop microscope

    with

    . QImaging QICAM colour camera

    for moderate

    . Fluorescence. Phase contrast. Motorised stage

    Moderate to high resolution fluorescence microscopy and imaging of histologically or histochemically stained specimens on slides. Motorised stage supports tiling and stitching of specimens.

    Leica

    Inverted

    Blue

    , Green

    , Red

    Fluorescence

  • Phase contrast
  • Long distance lenses

    • Leica DMIRB microscope

    with

    . Hamamatsu Orca NRB camera. Fluorescence. Phase contrast. Long distance lenses suitable for use with normal TC dishes and plates

    Good for moderate resolution fluorescence microscopy and phase contrast of fixed/live specimens on slides, or in dishes and plates with glass or plastic bottoms.

    Leica 2

    Inverted

    Blue

    , Cyan

    , Green

    Leica DMIRB microscope with Leica DFC7000T colour camera for

    , Red

    • Fluorescence
    • Phase contrast
    • LMC
    • Colour camera
    • Long distance lenses

    Leica DMIRB microscope. Leica DFC7000T colour camera. Fluorescence. Phase contrast. Leica Modulation Contrast (LMC). Long distance lenses suitable for use with normal TC dishes and plates

    Moderate to high resolution fluorescence microscopy and colour imaging of histologically or histochemically stained specimens on slides, or in dishes and plates with glass or plastic bottoms. Leica Modulation Contrast (LMC) lenses for brightfield contrast imaging that is less sensitive to the meniscus in well plates. Cyan filter cube for CFP.

    Confocal Microscopes

    Use a confocal microscope if your specimen in thick, has significant out-of-focus blur, or if you need to process, analyse or visualise it in 3D.

    Microscope Name

    Upright or Inverted?

    Objective Lenses

    Laser Lines

    		Features
    37°C Incubation
    Live Imaging

    Techniques

    Radiance458

    SPE

    Upright

    Suitable for coverslips and water dipping

    405, 488,

    514

    532,

    543, 635PossibleBio-Rad Microscience Radiance 2100 with Nikon 800 stand and three high-sensitivity GaAsP detectors for

    640

    		Leica TCS SPE with DM2500 stand. 1x PMT detector. DIC imaging.Perfusion chamber and dipping lenses on request.

    3D imaging of fixed specimens on slides.

    DIC.

    Live imaging

    of specimens in gasket sealed chambers or open dishes possible using a heated stage adapter and lens heater. Fluorescence recovery after photobleaching (FRAP).

    SPE

    Upright

    Suitable for coverslips and water dipping

    possible but not fast. Orthogonal (XZ) scans possible with the Z Galvo.

    SPE2

    Upright

    405, 488, 532, 640

    Possible

    		Leica TCS SPE with DM2500 stand
    for 3D imaging of fixed specimens on slides. Live imaging of specimens in dishes possible with water dipping lenses using Oko-lab stage adapter or Warner Instruments perfusion chamber. DIC (not dipping lenses). Spectral imaging.

    SPE2

    Upright

    Suitable for coverslips

    405, 488, 532, 640

    PossibleLeica TCS SPE with DM2500 stand for
    . 1x PMT detector. DIC imaging.Oko-Lab stage adapter and dipping lenses on request.

    3D imaging of fixed specimens on slides. Live imaging

    of specimens in dishes possible with water dipping lenses using Oko-lab stage adapter or Warner Instruments perfusion chamber. DIC (not dipping lenses). Spectral imaging

    possible but not fast. Orthogonal (XZ) scans possible with the Z Galvo.

    SPE3

    Inverted

    Suitable for coverslips

    405, 488, 561, 640

    No
    Leica TCS SPE
    version 2
    with DMI4000 stand. 1x PMT detector. DIC imaging. Motorised stage. Matrix screener module.No incubation equipment is available for this microscope.

    3D imaging of fixed specimens

    on

    in slides and

    in

    dishes. Fluorescence and DIC

    . Spectral Imaging. Motorised stage for multi

    on grids for CLEM. Multi-position

    acquisition

    imaging and tiling. Matrix Screener

    software

    for high content screening applications.

    SP5

    Inverted

    Suitable for coverslips

    405, 457, 476, 488, 495, 561, 594, 633

    Yes

    		Leica TCS SP5 with DMI6000B
    for 3D imaging of specimens on slides and live imaging of specimens in 35 mm dishes and Lab-Tek chambered coverslips using Pe-Con incubation equipment. Spectral imaging. DIC. Motorised stage for multi
    stand. 3x Hybrid and 1x PMT detectors. DIC. Motorised stage. FRAP and FRET wizards. Live Data Mode and Matrix Screener.Pe-Con stage-top incubator for 35 mm dishes.

    4D (3D+T) imaging of live and fixed specimens. CLEM. Multi-position acquisition and tiling. FRAP

    wizard. Förster Resonance Energy Transfer with FRET wizard. Experiment design using Live Data Mode. Matrix Screener for high content screening

    and FRET Acceptor Photobleaching or Sensitised Emission. Complex experiment design.

    Olympus FV1200Inverted
    Suitable for coverslips. Silicon lenses for live imaging

    405, 458, 488, 515, 559, 635

    Yes

    . Sim Scanner: 405 (pulsed for ablation), 440

    		Olympus FV1200 with IX83
    microscope
    stand.
    Live imaging using
    3x PMT, 2x GaAsP detectors. Second scanner for simultaneous imaging and ablation. Motorised stage. Silicon lenses. Time Controller module.Tokai-Hit stage
    adapter
    -top incubator with adaptors for dishes and chambered coverslips
    . Two spectral detectors, one PMT and two GaAsP detectors for high sensitivity imaging. DIC. Motorised stage. 32x and 60x silicon lenses for thick living specimens. Additional SIM scanner with 405 pulsed laser for ablation experiments and 440 nm laser for excitation of CFP. FRAP possible with or without SIM scanner
    .

    4D (3D+T) imaging of live and fixed specimens. High sensitivity imaging of green and red dyes with GaAsP detectors. Ablation of actin cortex. CLEM. Multi-position acquisition and tiling. Imaging thicker specimens. Complex experiment design.

    MultiphotonInverted405, 458, 488, 514, 561, 633. Tune-able Ti-Sapphire and OPOZeiss LSM 880 NLO with Axio Observer Z1 stand. 32 channel GaAsP, 2x PMT, 2x NDD and BiG2. Airyscan. Motorised stage. FCS/FCCS. Experiment Designer.Pe-Con cage incubator with CO2 chamber for 35 mm dishes4D (3D+T) imaging of live and fixed specimens. Multiphoton imaging and ablation. Fast spectral imaging and un-mixing. Super-resolution. Multi-position acquisition. Complex experiment design.

    Radiance

    Upright

    458, 488, 514, 543, 635

    		Bio-Rad radiance 2100 with Nikon E800 stand. 3x GaAsP detectors. Transmitted light contrast by DIC. Basic FRAP capability.Tokai-Hit stage heater and Bioptechs objective lens heater available on request.

    3D imaging of fixed specimens on slides.

    Spinning Disc Confocal Microscopes

    Use spinning disc microscopes for high-speed live confocal imaging of samples.

    Microscope Name

    Upright or Inverted?

    Objective Lenses

    Laser Lines

    		Key FeaturesLive Imaging

    Techniques

    Vox

    Inverted

    10x air to 100x oil (60x water immersion available)

    405, 488, 561, 640

    Perkin Elmer Ultraview Vox with Yokogawa CSU-X1 DSU and Nikon TiE stand. Hamamatsu C9100-13 EMCCD camera. Photokinesis Unit for FRAP. Motorised stage. Piezo focus.

    		Imaging of live specimens in 35 mm dishes, Lab-Tek chambered coverslips and Ibidi uSlides using Solent Scientific cage type incubation chamber.

    Sensitive and gentle high-speed live confocal imaging. This machine has a smaller field of view than the 3i but the image acquisition speed is higher. DIC. Multi-point and stitching. Good for FRAP. CLEM.

    3iInverted
    10x air to 100x oil (40x water immersion available)
    405, 440, 488, 514, 561, 640
    Imaging of live specimens in

    Yokogawa CSU-W1 spinning disc and Zeiss Axio Observer Z1 stand. Photometrics Prime 95b and Andor iXon 888 Life EMCCD cameras. Vector for FRAP. Motorised stage. High speed piezo focus

    		Oko-Lab cage incubator with CO2 chamber and adaptors for 35 mm dishes,
    Lab-Tek
    chambered coverslips and
    Ibidi uSlides using Oko-Lab cage type incubation chamber. DIC
    multi-well plates.Sensitive and gentle high-speed live confocal imaging. This machine has a larger field of view than the Vox but the image acquisition speed is not quite as high. Multi-point and stitching. FRAP.

    Time-lapse inverted microscope systems

    Microscope

    Objective Lenses
    Upright or Inverted?

    Fluorescence Colours

    Key Features

    CameraGreyscale high sensitivity CCD
    Live Imaging

    Techniques

    GFP1

    10x to 40x air and LWD (oil lenses available)

    Green and Red

    Inverted

    Green, Red, Far-Red

    Zeiss Axio Observer Z1 stand with Hamamatsu Orca Flash4.0 v2 sCMOS camera. Fully automated with motorised stage. Fluorescence and phase contrast.

    		Solent Scientific chamber with adaptor for multi-well plate. Other adaptors available on request.

    Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence.

    Stage adapters support Nunc and Mat-Tek multi-well plates and 35 mm Mat-Tek dishes. If you need to use another vessel or adapter you must contact Andrew Vaughan

    GFP2

    10x to 40x air and LWD (oil lenses available)

    GFP2

    		Inverted

    Cyan, Green, Red, Far-Red

    Zeiss Axiovert 200M stand with Hamamatsu Orca ER CCD camera. Fully automated with motorised stage. Fluorescence and phase contrast. Long distance lenses.

    		Solent Scientific chamber with adaptor for multi-well plate. Other adaptors available on request.

    Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence. Long distance lenses suitable for use with normal TC dishes and plates

    GFP3

    		Inverted

    Blue, Green and Red

    Greyscale high sensitivity CCD

    . Cyan and Yellow available on request.

    Nikon TiE stand. DS-Qi2 sCMOS camera with wide field of view. Fully automated with motorised stage. Perfect Focus System. Fluorescence and phase contrast. Long distance lenses.

    		Oko-Lab cage incubator with adaptors for multi-well plates, 35 mm dishes and chambered coverslips.

    Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence.

    Stage adapters support Nunc and Mat-Tek multi-well plates and 35 mm Mat-Tek dishes. If you need to use another vessel or adapter you must contact Andrew Vaughan

    N-STORM (coming soon)

    10x air to 100x oil TIRF lens

    Blue, Green, Red and Far-Red

    Greyscale ultra-high sensitivity EMCCD

    Optimally configured for high resolution and high sensitivity fluorescence microscopy of living specimens in XYZT for deconvolution, TIRF and super-resolution using N-STORM and dSTORM

    Long distance lenses suitable for use with normal TC dishes and plates. Perfect Focus System ensures that the sample remains in focus over long periods.


    Super-resolution Microscopes

    MicroscopeUpright or InvertedLaser LinesKey FeaturesLive ImagingTechniques
    ElyraInverted405, 488, 561, 642Zeiss Elyra PS.1 with Axio Observer Z1 stand. Andor iXon Du-897 EMCCD for PALM. PCO Edge sCMOS for SIM. Motorised stage and piezo focus. Single-band filter turret and optional dual-band filter turret for high speed dual channel imaging. 3D SIM and 3D PALM capabilities.Tokai-Hit stage-top incubator with adaptors suitable for 35 mm dishes and chambered coverslips.Structured Illumination Microscopy, PALM and dSTORM, Total Internal Reflection (TIRF) microscopy
    GFP4Inverted405, 488, 561, 642Visitech vt-iSIM and Nikon TiE stand. Hamamatsu Orca Flash4.0 v3 camera for vt-iSIM. Andor Zyla for wide-field imaging. Motorised stage and piezo focus.Oko-Lab cage incubator with adaptors for multi-well plates, 35 mm dishes and chambered coverslips.Instant SIM that gives a live structured illumination image without processing. Further resolution possible with deconvolution. Wide-field.
    N-STORMInverted405, 488, 561, 647Nikon N-STORM and Ti stand. Andor iXon Du-897 EMCCD camera for STORM and dSTORM. Astigmatic lens for 3D STORM. Zyla sCMOS camera for wide-field imaging.Solent Scientific cage incubator with adaptors for multi-well plates and 35 mm dishes.Stochastic Optical Reconstruction Microscopy (STORM) and direct STORM. TIRF microscopy. Wide-field time-lapse.
    STEDInverted405, WLLLeica TCS gSTED 3X with DMI6000B stand. 3x Hybrid and 2x PMT detectors. Motorised stage. High speed Z Galvo focus. 532, 660 and 775 nm depletion lasers for multi-colour STED. 3D STED capability.Life Imaging Services Box cage incubator system with adaptors for 35 mm dishes and chambered coverslipsMulti-colour STED and confocal microscopy. 3D STED. Tiling of large specimens.