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Like the Pearson Correlation Coefficient, this measures the degree to which the values of individual measurements of two channels change together either positively (correlated) or negatively (anti-correlated). A value of +1 indicates 100% correlation and −1 indicates 100% anti-correlation. A value of 0 indicates random correlation. Unlike the Pearson coefficient, the channel measurements do not have to be linearly related to give a correlation close to 1.
Manders Coefficients
The Manders Coefficients show the colocalised values from each channel as a ratio of the total fluorescence for that channel, so there is one coefficient for each channel.
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