MICROSCOPE |
---|
DMI6000, inverted |
STED RESOLUTION | |
---|---|
XY | ~ 50 nm |
Z | same as confocal |
IMAGING MODES |
---|
Laser scanning confocal imaging (multi-colour, multi-point, live cell) |
STED with 592 nm depletion, up to 3 colours |
LIGHT SOURCES | ||
---|---|---|
Light source | Type | Wavelengths [nm] |
Diode laser | Laser | 405 |
White light laser (WLL) | Laser | 470 - 670, tunable |
Depletion laser | Laser | 592 |
Leica EL6000 | Mercury metal halide bulb | ~ 300 - 700 (peaks at 365, 405, 436, 546, 579) |
OBJECTIVE LENSES | |||||
---|---|---|---|---|---|
Objective | Magnification | Immersion Medium | Numerical Aperture | Coverslip correction [mm] | Working Distance [mm] |
HCX PL APO CS | 10x | air | 0.4 | 0.17 | 2.2 |
HC PL FLUOTAR | 10x | air | 0.3 | none | 11 |
HC PL APO CS2 | 20x | IMM (water, glycerol, oil) | 0.75 | with or without coverglass/correction collar | 0.68 |
HCX APO LU-V-I | 20x | water | 0.5 | N/A | 35 |
HC FLUOTAR L VISIR | 25x | water | 0.95 | 0.17 | 24 |
HC PL APO CS2 | 40x | oil | 1.3 | 0.17 | 0.24 |
HC PL APO CS2 /1.4 (WD = 0.14 mm) | 63x | oil | 1.4 | 0.17 | 0.14 |
HC PL APO CS2 (dedicated STED objective) | 100x | oil | 1.4 | 0.17 | 0.13 |