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Warner Instruments perfusion equipment

Warner Instruments RC-37F - perfusion apparatus with two syringes

RC-37F insert attached to two syringes

The image above shows a Warner Instruments RC-37F insert for Falcon 35 mm dishes mounted in a Mat-Tek glass-bottomed dish. The glass-bottom dish is mounted in an MDA-1 mini-dish adapter for Warner RC-20 series chambers. The MDA-1 clamps are being used to hold the RC-37F in the dish. The MDA-1 is further mounted in an SA-20-MW-AL stage adapter for multi-wells. This will fit in any microscope specimen adapter designed for multi-well plates.

The RC-37F inlet and outlet ports are connected to short lengths of TY-50 Tygon 1/10” tubing which are in turn connected by TC-3 tubing connectors to SL-6 Luer stopcocks and standard plastic syringes. The TY-50 tubing can be cut to any length.

Warner Instruments equipment and consumables:

  • RC-37F insert for Falcon 35mm dishes (P/N 64-0348)
  • MDA-1 mini-dish adapter for RC-20 series (P/N 64-1675)
  • SA-20-MW-AL stage adapter for multi-wells (P/N 64-2416)
  • TY-50 Tygon 1/10” tubing (P/N 64-0167)
  • TC-3 tubing connectors (P/N 64-0164)
  • SL-6 Luer stopcocks (P/N 64-0165)

Warner Instruments RC-37F - perfusion apparatus with syringe manifold

RC-37 attached to syringe manifold

A. This image illustrates the overall appearance of a syringe manifold for supplying fluid to the RC-37F insert for 35 mm dishes. The flow is controlled using a pinch valve assembly that is controlled by switches on a VC-6 six channel valve controller.

B. A 50 ml syringe is mounted in the syringe manifold and connected to TY-50 Tygon 1/10” tubing by an SL6 Luer stopcock connected to a tubing adapter (TC-3)

C. A short piece of C-FLEX tubing is inserted into one of the pinch valves. This C-FLEX tubing is connected above and below the valve to lengths of TY-50 Tygon 1/10” tubing with tubing adapters (TC-3).

D. The RC-37F insert is mounted in a 35 mm Mat-Tek glass-bottomed dish. The dish is mounted in an MDA-1 mini-dish adapter for the RC-20 series of Warner chambers. The MDA-1 clamps are used to hold the RC-37F in the dish. The MDA-1 is further mounted in an SA-20-MW-AL stage adapter for multi-wells, which will fit in any microscope specimen adapter designed for multi-well plates. The RC-37F inlet port is connected to an MP-2 manifold using short pieces of PE-160 polyethylene tubing. The outlet port is attached to a short piece of C-FLEX tubing. Warner Instruments recommend attaching the outlet to a vacuum line, but a peristaltic or syringe pump might also work.

Warner Instruments equipment and consumables:

  • RC-37F insert for Falcon 35mm dishes (P/N 64-0348)
  • MDA-1 mini-dish adapter for RC-20 series (P/N 64-1675)
  • SA-20-MW-AL stage adapter for multi-wells (P/N 64-2416)
  • TY-50 Tygon 1/10” tubing (P/N 64-0167)
  • TC-3 tubing connectors (P/N 64-0164)
  • SL-6 Luer stopcocks (P/N 64-0165)
  • CFL-6 C-FLEX tubing 1/32" (P/N 64-0166)
  • PE-160 tubing, 1.57 OD x 1.41 ID (P/N 64-0755)
  • MP-2 manifold (P/N 64-0206)
  • VC-6 six channel valve controller with pinch valve assembly
  • SH-6/60 Syringe holder with six 60 cc syringes (P/N 64-0163)
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