Instant SIM (iSIM)

Instant SIM is a technique pioneered by York et al and developed commercially by Visitech. The concept uses the Image Scanning Microscopy (ISM) approach described mathematically by Colin Sheppard in 1988. The technology creates a super-resolution image in analogue form using optics alone so that the image produced by the camera requires no reconstruction, although deconvolution improves the resolution further. iSIM and other image scanning microscopy techniques approach the theoretical maximum resolution possible using confocal microscopy.

Here RPE1 cells have been labelled using an antibody against the mitochondrial protein Tom20 with an Oregon Green labelled secondary antibody.

Airyscan

Airyscan is another ISM technique developed by Carl Zeiss and implemented on the company's LSM confocal microscopes

Structured Illumination Microscopy (SIM)

In Structured Illumination Microscopy high frequency information that is out of the bandwidth of the diffraction-limited microscope optics is shifted into the bandwidth by convolution with a known frequency; normally a grid pattern projected using a diffraction grating is spatial light modulator. The grating pattern must be shifted laterally and rotated in order to sample high frequencies in all orientations, which means 15 to 25 images must be taken to make one super-resolution image.