Start-up Procedure
Turn on the computer and login to the User account. In no particular order turn on the QICAM camera (there's a switch on its back panel), the Optiscan II Control Box on the shelf, the microscope stand (green button on the side of the stand) and the fluorescent lamp if you need it.
Motorised Components
- Open the transmitted light shutter by pressing the button 'TL Shut' on the Orbit keypad
- Focus using the knob on the side of the joystick - NOT the microscope focus knob. You can toggle between coarse, fine and extra fine focus speeds by pressing the Focus Speed button on the joystick. The orange tape on the side of the microscope behind the stage acts as a focus guide. Line the two pieces up and you should be in focus
- Use the joystick to move the stage and adjust its speed with the Stage Speed button
- Use the blue, green and red buttons on the Orbit keypad to change fluorescence illumination. These buttons MUST ONLY be used in combination with the second filter set in the microscope fluorescence slider ('Separate BGR')
- The Shutter button blocks the fluorescence light path. Don't use any of the other buttons.
Using Volocity
Open Volocity and create or open a library. The light paths labelled 1 to 9 at the top of the video controls palette are:
- Bright Field, 1x binning, colour
- Blue Fluorescence, 1x binning, colour
- Green Fluorescence, 1x binning, colour
- Red Fluorescence, 1x binning, colour
- Close Shutters (cuts off all light)
- Bright Field, 2x binning, greyscale
- Blue Fluorescence, 2x binning, greyscale
- Green Fluorescence, 2x binning, greyscale
- Red Fluorescence, 2x binning, greyscale