Leica

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Objective lenses

Magnification	Immersion	Designation	NA	Contrast Technique	Coverslip Correction (mm)	WD (mm)
10x	Air	N Plan	0.25	Ph1	None	17.6
20x	Air	N Plan LWD	0.4	Ph1	0 - 2	3.2 - 1.9
40x	Air	N Plan LWD	0.55	Ph2	0 - 2	3.3 - 1.9
20x	Air	N Plan LWD	0.4	LMC	0 - 2	3.2 - 1.9
40x	Air	N Plan LWD	0.55	LMC	0 - 2	3.3 - 1.9

Filter Position	Filter Set	Excitation Filter	Dichroic Mirror	Emission Filter	Fluorophores
1	Leica A4	360/40	400	470/40	DAPI, Hoechst
2	Leica GFP	470/40	500	525/50	FITC, GFP
3	Leica N2.1	515 - 560	580	LP 590	Cy3, TRITC
4	Blank	N/A	N/A	N/A	Transmitted Light

Magnification	Binning Factor	100 pixels =	1 pixel =
10x	1	106.34 µm	1063.4 nm
20x	1	53.17 µm	531.7 nm
40x	1	26.59 µm	265.9 nm
10x	2	212.68 µm	2126.8 nm
20x	2	106.34 µm	1063.4 nm
40x	2	53.17 µm	531.7 nm