Microscope Stand Information
Unless otherwise stated the microscope objective lenses require specimens to be mounted on a #1.5 glass coverslip (170 microns) or in a chamber/dish with a coverslip bottom of the same thickness.
Widefield
Use a widefield microscope if your specimen is thin and relatively free of out-of focus blur or you don't need to optically section it for processing, analysis and visualisation in 3D.
Microscope |
Upright or Inverted? |
Objective Lenses |
Fluorescence Colours |
Camera |
Automation |
Techniques |
|---|---|---|---|---|---|---|
Axioplan |
Upright |
10x air to 100x oil |
Blue, Green, Red, Far-Red |
Greyscale high sensitivity CCD |
Manual |
Good for high sensitivity and high resolution fluorescence microscopy and differential interference contrast (DIC) of fixed specimens on slides |
Axio Imager |
Upright |
10x air to 100x oil |
Blue, Green, Red, Far-Red (Cyan and Yellow available on request) |
Greyscale high sensitivity CCD |
Manual |
Good for high sensitivity and high resolution fluorescence microscopy and phase contrast of fixed specimens on slides |
Axioskop 3 |
Upright |
10x air to 100x oil |
Blue, Green, Red |
Colour CCD |
Motorised |
Good for moderate to high resolution fluorescence microscopy and colour imaging of histologically or histochemically stained specimens on slides. Supports tiling and stitching. |
Inverted |
10x air to 40x LWD* |
Blue, Green, Red |
Greyscale high sensitivity CCD |
Manual |
Good for moderate resolution fluorescence microscopy and phase contrast of fixed/live specimens on slides or in dishes and plates with glass or plastic bottoms |
|
Leica 2 |
Inverted |
10x air to 40x LWD* |
Blue, Green, Red |
Colour CCD |
Manual |
Good for moderate resolution fluorescence microscopy and colour imaging of histologically or histochemically stained specimens on slides or in dishes and plates with glass or plastic bottoms |
Confocal
Use a confocal if your specimen in thick, has significant out-of-focus blur, or if you need to process, analyse or visualise it in 3D.
Microscope |
Upright or Inverted? |
Objective Lenses |
Lasers |
Detectors |
Simultaneous/Sequential |
Manual/Automated Stage |
Climate Control |
Techniques |
|---|---|---|---|---|---|---|---|---|
Radiance |
Upright |
10x air to 60x oil (water dipping lenses available) |
Argon, 543, 635 |
3x GaAsP |
Simultaneous and Sequential |
Manual |
Available (heat only) |
Imaging of specimens on slides and in gasket sealed chambers or open dishes using dipping lenses. DIC. FRAP. |
SPE |
Upright |
10x air to 63x oil (water dipping lenses available) |
405, 488, 532, 640 |
1x PMT |
Sequential only |
Manual |
Available |
Imaging of specimens on slides and live specimens in Warner Instruments perfusion chamber using dipping lenses. DIC. Spectral imaging. |
SPE |
Upright |
10x air to 63x oil (water dipping lenses available) |
405, 488, 532, 640 |
1x PMT |
Sequential only |
Manual |
Available |
Imaging of specimens on slides and live specimens in open dishes using Oko-Lab incubation equipment and dipping lenses. DIC. Spectral imaging. |
SPE3 |
Inverted |
10x air to 63x oil |
405, 488, 561, 640 |
1x PMT |
Sequential only |
Automated |
No |
Imaging of specimens on slides and in dishes. DIC. Spectral Imaging. Multi-point and stitching. Matrix Screener. |
SP5 |
Inverted |
10x air to 63x oil (63x glycerol available) |
405, Argon, 561, 594, 633 |
1x PMT and 3x Hybrids |
Simultaneous and Sequential |
Automated |
Available |
Imaging of specimens on slides and in 35 mm dishes and Lab-Tek chambered coverslips using Pe-Con incubation equipment. Spectral imaging. DIC. Multi-point and stitching. FRAP. FRET. Live Data Mode. Matrix Screener. |
Vox |
Inverted |
10x air to 100x oil (60x water immersion available) |
405, 488, 561, 640 |
1x EMCCD camera |
Sequential only |
Automated |
Available |
Imaging of live specimens in 35 mm dishes, Lab-Tek chambered coverslips and Ibidi uSlides using Solent Scientific cage type incubation chamber. DIC. Multi-point and stitching. FRAP. |
Time-lapse inverted microscope systems
Microscope |
Objective Lenses |
Fluorescence Colours |
Camera |
Techniques |
|---|---|---|---|---|
GFP1 |
10x to 40x air and LWD (oil lenses available) |
Green and Red |
Greyscale high sensitivity CCD |
Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence. Stage adapters support Nunc and Mat-Tek multi-well plates and 35 mm Mat-Tek dishes. If you need to use another vessel or adapter you must contact Andrew Vaughan |
GFP2 |
10x to 40x air and LWD (oil lenses available) |
Blue, Green and Red |
Greyscale high sensitivity CCD |
Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence. Stage adapters support Nunc and Mat-Tek multi-well plates and 35 mm Mat-Tek dishes. If you need to use another vessel or adapter you must contact Andrew Vaughan |
N-STORM (coming soon) |
10x air to 100x oil TIRF lens |
Blue, Green, Red and Far-Red |
Greyscale ultra-high sensitivity EMCCD |
Optimally configured for high resolution and high sensitivity fluorescence microscopy of living specimens in XYZT for deconvolution, TIRF and super-resolution using N-STORM and dSTORM. |