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  1. The FV10-ASW 4.2 confocal image acquisition software is very modular and the main panels are:

    1. Acquisition Setting: allows you to set up the parameters for the confocal image acquisition
    2. Image Acquisition Control: here you can set up the lightpath for your experiment and start an image preview or the image acquisition
    3. Live View: shows as live preview of your sample
    4. Data Manager: displays properties of selected image files
    5. Explorer: here you can access saved files and load acquisition parameters of previous experiments

  2. First you need to set up the light path including lasers and detectors using the Dye List menu. This can be accessed by clicking the on the Dye List icon located on the right hand side of the Image Acquisition Control panel.


     
  3. In the Dye List menu (shown below) drag the dyes you would like to use into the Selected Dyes box or double-click to select them. If you want to remove any dyes you can drag them out of the list individually or click clear all to remove all dyes and start over. Click Apply and the lightpath for the selected dye combination will be configured automatically (includes activation of lasers and selection of detectors and filters)



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