DIVE lasers
- Andrew Vaughan
Owned by Andrew Vaughan
The DIVE has the following visible solid state lasers for confocal microscopy.
Wavelength | Colour | Example dyes |
|---|---|---|
| 405 | DAPI, Hoechst | |
| 448 | CFP | |
| 488 | GFP, Alexa 488 | |
| 514 | YFP | |
| 561 | mCherry, Alexa 568 | |
| 638 | Cy5, Alexa 647 |
The infrared laser for multiphoton microscopy is a Coherent Discovery NX with a fixed line at 1040 nm that can be used for red dyes such as tdTomato and mCherry, and a tuneable line that can be adjusted between 660 nm and 1320 nm. The fixed and tuneable lines can be used simultaneously.