Objective Lenses
Magnification | Immersion | Designation | NA | Contrast Technique | Coverslip Correction (mm) | WD (mm) |
|---|---|---|---|---|---|---|
10X | Air | N Plan | 0.25 | Ph1 | None | 17.6 |
20X | Air | HCX PL Fluotar LWD | 0.4 | Ph1 | 0 - 2 | 6.9 |
40X | Air | N Plan LWD | 0.55 | Ph2 | 0 - 2 | 3.3 - 1.9 |
10X | Air | C Plan | 0.22 | LMC | None | 7.8 |
20X | Air | N Plan LWD | 0.4 | LMC | 0 - 2 | 3.2 - 1.9 |
40X | Air | N Plan LWD | 0.55 | LMC | 0 - 2 | 3.3 - 1.9 |
NA = Numerical Aperture, WD = Working Distance, LWD = Long Working Distance, LMC = Leica Modulation Contrast
Fluorescence Filter Sets
Filter Position | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Fluorophores |
|---|---|---|---|---|---|
1 | Leica A4 | BP 360/40 | 400 | BP 470/40 | DAPI, Hoechst |
2 | Leica GFP | BP 470/40 | 500 | BP 525/50 | FITC, GFP |
3 | Leica N2.1 | BP 515-560 | 580 | LP 590 | Cy3, TRITC |
4 | Blank | N/A | N/A | N/A | Transmitted Light |