Axio Imager
Location: MRC Building, First Floor, Room 1.19.
The Zeiss Axio Imager upright microscope is ideal for viewing slide-mounted specimens using phase contrast or fluorescence microscopy. It has a QImaging Retiga greyscale camera suitable for low light level imaging of fluorescent specimens. The objective lenses are all designed for high resolution observation of specimens mounted under standard #1.5 coverslips (170 µm). All microscope controls are manual apart from camera image capture. There are curretnly three image acquisition software packages installed on the computer: Openlab (obsolete - updates no longer available); Volocity and µManager.
To request training, report problems or ask for help please email lmcbimage@lmcb.mcbl.ucl.ac.uk.
Objective lenses on Axio Imager
Magnification |
Immersion |
Designation |
NA |
Contrast |
Coverslip Correction (mm) |
|---|---|---|---|---|---|
10X |
Air |
EC Plan Neofluar |
0.3 |
Ph1 |
0.17 |
20X |
Air |
EC Plan Neofluar |
0.5 |
Ph2 |
0.17 |
40X |
Oil |
EC Plan Neofluar |
1.3 |
Ph3 |
0.17 |
63X |
Oil |
Plan Apochromat |
1.4 |
Ph3 |
0.17 |
100X |
Oil |
EC Plan Neofluar |
1.3 |
Ph3 |
0.17 |
Fluorescence filter sets on Axio Imager
Filter Turret |
Turret Position |
Dyes |
Excitation Filter |
Dichroic Mirror |
Emission Filter |
|---|---|---|---|---|---|
1 |
DAPI |
350/50 |
400LP |
460/50 |
|
2 |
DIC |
N/A |
N/A |
Analyser |
|
3 |
Empty |
N/A |
N/A |
N/A |
|
4 |
GFP |
470/40 |
495LP |
525/50 |
|
5 |
Texas Red |
560/40 |
585LP |
630/75 |
|
6 |
Cy5 |
620/60 |
660LP |
665LP |