Image Acquisition using the Radiance 2100
The LaserSharp 2000 software supports user profiles. Double-click the LaserSharp 2000 icon; select your name from the drop-down list that appears and enter your password.
The LaserSharp 2000 interface consists of a main menu and three windows: the Experiment Browser; the Image Display Window and the Control Panel. The full interface can be seen here.
First Steps
Load a Method
Settings for acquiring single and multi-channel datasets, along with more exotic configurations, are stored in the form of methods. Preset methods can be accessed by clicking on 'Methods' in the main menu.
Recently used methods can be selected from the drop-down list. If the method you want isn't there, click on 'Methods...' to view an expanded list.
Click a method on this list and click the 'Load the Selected Method' icon (below) to configure the system with your desired settings.
Set the Objective Lens
Select the correct objective lens from the drop-down window in the Control Panel. This will ensure that subsequent images are correctly spatially calibrated.