Microscope Stand Information
Unless otherwise stated the microscope objective lenses require specimens to be mounted on a #1.5 glass coverslip (170 microns) or in a chamber/dish with a coverslip bottom of the same thickness.
Widefield
Use a widefield microscope if your specimen is thin and relatively free of out-of focus blur or you don't need to optically section it for processing, analysis and visualisation in 3D.
Microscope Name | Upright or Inverted? | Objective Lenses | Fluorescence Colours | Techniques |
|---|---|---|---|---|
Axioplan | Upright for slides | Up to 100x oil | Blue, Green, Red, Far-Red | Zeiss Axioplan 2 microscope with QImaging QClick camera for high sensitivity and high resolution fluorescence microscopy and differential interference contrast (DIC) of fixed specimens on slides |
Axio Imager | Upright for slides | Up to 100x oil | Blue, Green, Red, Far-Red (Cyan and Yellow available on request) | Zeiss Axio Imager A1 microscope with QImaging Retiga EXi for high sensitivity and high resolution fluorescence microscopy and phase contrast of fixed specimens on slides |
Axioskop 3 | Upright for slides | Up to 100x oil | Blue, Green, Red | Zeiss Axioskop microscope with QImaging QICAM colour camera for moderate to high resolution fluorescence microscopy and imaging of histologically or histochemically stained specimens on slides. Motorised stage supports tiling and stitching of specimens. |
Inverted for TC dishes | Long distance air lenses up to 40x | Blue, Green, Red | Leica DMIRB microscope with Hamamatsu Orca NRB camera. Good for moderate resolution fluorescence microscopy and phase contrast of fixed/live specimens on slides or in dishes and plates with glass or plastic bottoms | |
Leica 2 | Inverted for TC dishes | Long distance air lenses up to 40x | Blue, Green, Red | Leica DMIRB microscope with Leica DFC7000T colour camera for high resolution fluorescence microscopy and colour imaging of histologically or histochemically stained specimens on slides or in dishes and plates with glass or plastic bottoms |
Confocal
Use a confocal if your specimen in thick, has significant out-of-focus blur, or if you need to process, analyse or visualise it in 3D.
Microscope Name | Microscope Model | Upright or Inverted? | Objective Lenses | Lasers | Detectors | Simultaneous/Sequential | Manual/Automated Stage | Climate Control | Techniques |
|---|---|---|---|---|---|---|---|---|---|
Radiance | Bio-Rad Microscience Radiance 2100 with Nikon E800 stand | Upright | 10x air to 60x oil (water dipping lenses available) | Argon, 543, 635 | 3x GaAsP | Simultaneous and Sequential | Manual | Available (heat only) | Imaging of specimens on slides and in gasket sealed chambers or open dishes using dipping lenses. DIC. FRAP. |
SPE | Leica TCS SPE with DM2500 stand | Upright | 10x air to 63x oil (water dipping lenses available) | 405, 488, 532, 640 | 1x PMT | Sequential only | Manual | Available | Imaging of specimens on slides and live specimens in Warner Instruments perfusion chamber using dipping lenses. DIC. Spectral imaging. |
SPE | Leica TCS SPE with DM2500 stand | Upright | 10x air to 63x oil (water dipping lenses available) | 405, 488, 532, 640 | 1x PMT | Sequential only | Manual | Available | Imaging of specimens on slides and live specimens in open dishes using Oko-Lab incubation equipment and dipping lenses. DIC. Spectral imaging. |
SPE3 | Leica TCS SPE version 2 with DMI4000 stand | Inverted | 10x air to 63x oil | 405, 488, 561, 640 | 1x PMT | Sequential only | Automated | No | Imaging of specimens on slides and in dishes. DIC. Spectral Imaging. Multi-point and stitching. Matrix Screener. |
SP5 | Leica TCS SP5 with DMI6000B stand | Inverted | 10x air to 63x oil (63x glycerol available) | 405, Argon, 561, 594, 633 | 1x PMT and 3x Hybrids | Simultaneous and Sequential | Automated | Available | Imaging of specimens on slides and in 35 mm dishes and Lab-Tek chambered coverslips using Pe-Con incubation equipment. Spectral imaging. DIC. Multi-point and stitching. FRAP. FRET. Live Data Mode. Matrix Screener. |
Vox | Perkin Elmer Ultraview Vox with Nikon TiE stand | Inverted | 10x air to 100x oil (60x water immersion available) | 405, 488, 561, 640 | 1x EMCCD camera | Sequential only | Automated | Available | Imaging of live specimens in 35 mm dishes, Lab-Tek chambered coverslips and Ibidi uSlides using Solent Scientific cage type incubation chamber. DIC. Multi-point and stitching. FRAP. |
| 3i | Yokogawa CSU-W1 with Zeiss Axio Observer Z1 stand | Inverted | 10x air to 100x oil (40x water immersion available) | 405, 440, 488, 514, 561, 640 | 1x EMCCD camera and 1x sCMOS camera | Sequential only | Automated | Available | Imaging of live specimens in 35 mm dishes, Lab-Tek chambered coverslips and Ibidi uSlides using Oko-Lab cage type incubation chamber. DIC. Multi-point and stitching. FRAP. |
Time-lapse inverted microscope systems
Microscope | Objective Lenses | Fluorescence Colours | Camera | Techniques |
|---|---|---|---|---|
GFP1 | 10x to 40x air and LWD (oil lenses available) | Green and Red | Greyscale high sensitivity CCD | Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence. Stage adapters support Nunc and Mat-Tek multi-well plates and 35 mm Mat-Tek dishes. If you need to use another vessel or adapter you must contact Andrew Vaughan |
GFP2 | 10x to 40x air and LWD (oil lenses available) | Blue, Green and Red | Greyscale high sensitivity CCD | Optimally configured for 'screening' of multiple positions in multi-well plates and other vessels at low to moderate resolution and high sensitivity using phase contrast and fluorescence. Stage adapters support Nunc and Mat-Tek multi-well plates and 35 mm Mat-Tek dishes. If you need to use another vessel or adapter you must contact Andrew Vaughan |
N-STORM (coming soon) | 10x air to 100x oil TIRF lens | Blue, Green, Red and Far-Red | Greyscale ultra-high sensitivity EMCCD | Optimally configured for high resolution and high sensitivity fluorescence microscopy of living specimens in XYZT for deconvolution, TIRF and super-resolution using N-STORM and dSTORM. |