Leica
Objective lenses
Magnification |
Immersion |
Designation |
Contrast Technique |
Coverslip Correction (mm) |
WD (mm) |
|
---|---|---|---|---|---|---|
10x |
Air |
N Plan |
0.25 |
Ph1 |
None |
17.6 |
20x |
Air |
N Plan LWD |
0.4 |
Ph1 |
0 - 2 |
3.2 - 1.9 |
40x |
Air |
N Plan LWD |
0.55 |
Ph2 |
0 - 2 |
3.3 - 1.9 |
20x |
Air |
N Plan LWD |
0.4 |
LMC |
0 - 2 |
3.2 - 1.9 |
40x |
Air |
N Plan LWD |
0.55 |
LMC |
0 - 2 |
3.3 - 1.9 |
Fluorescence filter sets
Filter Position |
Filter Set |
Excitation Filter |
Dichroic Mirror |
Emission Filter |
Fluorophores |
---|---|---|---|---|---|
1 |
Leica A4 |
360/40 |
400 |
470/40 |
DAPI, Hoechst |
2 |
Leica GFP |
470/40 |
500 |
525/50 |
FITC, GFP |
3 |
Leica N2.1 |
515 - 560 |
580 |
LP 590 |
Cy3, TRITC |
4 |
Blank |
N/A |
N/A |
N/A |
Transmitted Light |
Camera Calibrations
Magnification |
100 pixels = |
1 pixel = |
|
---|---|---|---|
10x |
1 |
106.34 µm |
1063.4 nm |
20x |
1 |
53.17 µm |
531.7 nm |
40x |
1 |
26.59 µm |
265.9 nm |
10x |
2 |
212.68 µm |
2126.8 nm |
20x |
2 |
106.34 µm |
1063.4 nm |
40x |
2 |
53.17 µm |
531.7 nm |