Lasers
Excitation Lasers | ||
---|---|---|
Wavelength | Type | Fluorophores |
405 nm | CW | DAPI, Hoechst, etc |
White Light Laser† | Pulsed | All visible dyes from green to far red |
STED Depletion Lasers‡ | ||
592 nm | CW | STED with green dyes |
660 nm | CW | STED with red dyes |
775 nm | Pulsed | STED with far red dyes |
† The White Light Laser (WLL) is a super-continuum that can be tuned from 470 nm to 670 nm and can therefore be used to excite all visible dyes.
‡ The depletion lasers are used to increase resolution by stimulating re-emission of photons. They are not used for excitation and are only used in STED.
Objective lenses
Objective lenses | |||||
---|---|---|---|---|---|
Magnification | Type | Immersion | NA | Working Distance (mm) | Coverslip correction |
10x | HC PL FLUOTAR | Air | 0.3 | 11 | 0.17 |
20x | HC PL APO CS2 | Imm (Oil, Glycerol and Water) | 0.75 | 0.68 | 0/0.17 |
25x | HC FLUOTAR L VISIR | Water | 0.95 | 2.4 | 0.17 |
40x | HC PL APO CS2 | Oil | 1.3 | 0.24 | 0.17 |
63x | HC PL APO CS2 | Oil | 1.4 | 0.14 | 0.17 |
100x | HC PL APO CS2 (dedicated STED objective) | Oil | 1.4 | 0.13 | 0.17 |
NOTE: There is only space for 6 objective lenses on the objective turret, so if the lens you would like to use is not on there please contact lmcb-lm-help@ucl.ac.uk
Microscope stand specifications and filters
The microscope is a fully motorised DMI 6000 CS inverted model with AFC auto focus, a Märzhäuser motorised scanning stage and a high speed Z Galvo focus in addition to the stand's internal focus motor.
Filter sets | ||||
---|---|---|---|---|
Name | Excitation Filter | Dichroic Mirror | Emission Filter | Typical Fluorophores |
I3 | BP 450-490 | 510 | LP 515 | FITC, GFP, Alexa 488 |
N2.1 | BP 515-560 | 580 | LP 590 | TRITC, RFP, Alexa 568 |
POL | - | - | - | Polariser for DIC |
BF | Empty | Empty | Empty | Confocal or Brightfield |
A | BP 340-380 | 400 | LP 425 | DAPI, Hoechst |
Empty | - | - | - | Spare position |
DETECTORS | |
---|---|
3x HyD, high sensitivity, 3 operating modes (standard, counting, BrightR) + time-gating | |
3x PMT, one of which for transmitted light imaging |
A
INCUBATION: The microscope is fully enclosed and equipped with The Cube chamber heater and The Brick CO2 gas mixer.
SOFTWARE | |
---|---|
Acquistion | LAS X |
Deconvolution | Huygens Professional Version 15.10.1p2 |
A
ADDITIONAL INFORMATION:
- resonant scanner (8000 Hz) for fast imaging
- anti-vibration optical table
- fully enclosed
- autofocus
- motorized XY stage
- Z-galvo