Location: MRC Building. Room G.10.
The CMOS camera can be used to simultaneously acquire images from two fluorescence channels. The beam splitter sends far red light to one channel and blue, green or orange light to the other. Ideal fluorophores are Cy3 and Cy5, or dyes with similar spectra. Note that the system is optimised for the Cy3/Cy5 FRET pair and it is not possible to image samples labelled with both green and red fluorophores. Advantages of the CMOS camera are speed and field of view.
Use for:
- Simultaneous dual-channel single molecule localisation microscopy
- smFRET
- Single molecule tracking
- Microfluidics for single molecule imaging