Super-Resolution Microscopy

Glox Switching Buffer

The Glox buffer formulation is adapted from the following publication:

Metcalf DJ, Edwards R, Kumarswami N, Knight AE. Test samples for optimizing STORM super-resolution microscopy. J Vis Exp 2013 Sep 6;(79):50579. doi: 10.3791/50579

To make 2.2 ml final volume, which will fill one well of a 4-well ibidi µ-Slide.

• 110 µl Solution A
• 880 µl Solution B
• 220 µl Solution C
• 990 µl PBS

Solution A (Enzyme Solution)

• 10 µl catalase
• 20 µl 1M TCEP
• 2 ml glycerol
• 125 µl 1M KCl
• 100 µl 1M Tris-Cl pH 7.5
• 50 mg glucose oxidase
• Top up to 5 ml with distilled water

Dispense into 50 µl aliquots and store frozen at -20°C.

Solution B (Glucose Solution)

• 4 g glucose
• 4 ml glycerol
• 36 ml distilled water

Dispense into 400 µl aliquots and store at -20°C.

Solution C (Reducing Agent Solution)

• 113.6 mg MEA-HCl
• 1 ml distilled water

Use fresh or make 100 µl aliquots for storage at -20°C