...

You can edit the imaging setup after loading the Smart Setup configuration, or alternatively you can create an imaging setup manually from scratch without using Smart Setup.

Detectors

The microscope has three main detectors (internal aka descanned detectors) suitable for both confocal and multiphoton imaging, and a further four for multiphoton only (non descanned detectors).

Two of the detectors (Ch1 and Ch2) are high sensitivity GaAsP detectors and a third (ChS1) is a Zeiss Quasar detector made up of a 32-channel GaAsP array. This array can be configured as multiple detectors or can be used in the Lambda and Online Fingerprinting modes to generate a spectral image of the sample.

You can use Ch1, Ch2 or up to 32 individual channels from ChS1 (or some combination of these) as detectors.

You can change the broadness of the filter band by dragging it or clicking the down arrow to type in numbers.

You can add new ChS detectors using the + button and delete them using the - button.

Primary and Secondary Dichroic Mirrors

Select suitable primary dichroic mirrors for the visible and invisible lines (405 or multiphoton).

If the Mirror is in position the light will be sent to the Ch1, Ch2 and ChS detectors. If another position is selected then some portion of the light (up to 100%) will be sent to the Airyscan detector. See Airyscan for details.

The Reflector should be in the Rear position unless you are using the NDDs for multiphoton imaging.

Acquisition Mode

• Expand Acquisition Mode. In here you can change a variety of parameters but the main ones are the scan settings:

...