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- Turn on the "Main switch" on the keypad.
- Turn on "Systems/PC" on the keypad.
- Wait for computer to boot up and enter the Windows account credentials if prompted.
- Turn on the "Components" switch on the keypad.
- Double-click the Zen Black icon on the desktop.
- Click the "Start System" button.
If CO2 Module S and TempModule S are off, you will see messages warning you about this in the Error Log in the bottom left corner. This is normal and can be ignored.
Click the" Acquisition" tab and expand the "Laser" menu. Turn on the lasers you need. (Some of the lasers might show a red background initially, which means it is not ready to be used. This will go away after a couple of minutes when the laser is warmed up).
Locating your Specimen
- Click the "Locate" tab and expand the "Microscope Control" drop-down menu.
- Select the objective lens you'd like to use - either via the menu in locate', or via the touchscreen controller next to the screen.
- Select a suitable fluorescence filter. Only the first three should be used; the rest are special positions for the confocal and multiphoton.
- Put your specimen on the microscope and open either the transmitted light shutter or the fluorescence shutter depending on which mode you want to use to find your sample.
- Turn on the lamp by clicking its icon and pressing the On button.
- Open the fluorescence shutter to let light through.
- When your specimen is in focus, close the shutter and click the "Acquisition" tab.
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