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• Click the "Locate" tab and expand the "Microscope Control" drop-down menu.
• Select the objective lens you'd like to use - either via the menu in locate, or via the touchscreen controller next to the screen.
• Select a suitable fluorescence filter. Only the first three should be used for fluorescence; the rest are special positions for the confocal and multiphoton, but you can use BS-MP positions 4 and 5 for transmitted light.
• Put your specimen on the microscope and open either the transmitted light shutter or the fluorescence shutter depending on which mode you want to use to find your sample.
• Turn on the lamp by clicking its icon and pressing the On button.

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• When your specimen is in focus, close the shutter and click the "Acquisition" tab.

Imaging Setup

Smart Setup

The easiest way to set up imaging conditions is to use Smart Setup.

Click on the Smart Setup button and add the dyes you want to image.

Choose from Fastest, Best Signal or Smartest proposals. Basically, Fastest will be simultaneous (one Track), Best Signal will capture all channels separately, moving the filters each time to optimise signal (which is slow). Smartest optimises the filter positions so that they don't have to move, but still captures the channels sequentially.

The applicable lasers will automatically turn on and the filters will automatically move into the positions programmed for that dye combination and configuration.

Manual Setup

• Expand Imaging Setup
• Choose LSM and Channel
• (Non Descanned is for multiphoton only)
• (Lambda Mode and Online Fingerprinting are for spectral imaging)
• Use Smart Setup if you want to easily set up your experimental settings (e.g. filter and detector combinations) depending on your dyes.

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