Before you start

Heating and CO2 for the incubator

The CO2 Module S and TempModule S are normally switched off at the back. If temperature and/or CO2 control is required, these must be switched on.

Coherent Vision II multiphoton laser

If you need the Coherent Chameleon Vision II Ti Sapphire laser for multiphoton imaging or ablation, you must turn the key on the Coherent power supply from Standby to On and wait for the power to ramp up until the Status says OK (takes a minute or two). You can continue with the start-up of the rest of the system while the laser ramps up.

Main switch laser safety key

The key on the side of the keypad must always be left in the horizontal position (see image below), even when the system is powered down. However, occasionally I find that it has been turned to the vertical position, which means it is switched off. Make sure it is in the horizontal position, otherwise the lasers and anything else connected to the "Components" switch will not come on. 

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If this happens, you should quit Zen, turn on the key and then open Zen again.

Switching On

• Turn on the "Main switch" on the keypad
• Turn on "Systems/PC" on the keypad
• Wait for computer to boot up and log in to LSM-User account
• Turn on the "Components" switch on the keypad. 
• Click the "Start System" button

• Click the" Acquisition" tab and expand the "Laser" menu. Turn on the lasers you need.  (Some of the lasers might show a red background initially, which means it is not ready to be used.  This will go away after a couple of mintues when the laser is warmed up)

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