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Instructions Part 2


This document is an introduction to the layout of the Vox user interface and can also be used as a reference guide. The user interface is similar on all microscope systems that use Volocity but the details vary depending on the installed devices. For example, Sensitivity is a control that you will only see if there is an EMCCD camera installed on the system. More information about general aspects of Volocity as an acquisition software can be found in the Volocity User Guide or on the support section of the PerkinElmer Cellular Imaging website.

Please click on the images in the text to expand them.

Libraries and the Video Preview icon

When you first open Volocity you will be prompted to either open an existing Library or create a new Library. Libraries are databases where your images are stored and they must be created if you want to do anything in Volocity. You can create a new library for each experiment or have a number of experiments in a single library. I would probably recommend the former approach in most cases since if a large library with a lot of data in it is corrupted then all that data will be lost.

Libraries

Once you've created a library you can click on the Video Preview icon on the left of the screen. There's also a FRAP Preview icon but this is for use purely in FRAP experiments.

Video Preview icon

Video Preview

The video preview window consists of three sections: The Library on the left where the Video and FRAP Preview icons are and where all captured images and other items will appear as icons; the Video Preview in the middle that shows a live preview of data from the camera; and the Device Controls on the right.

Vox user interface

Next: Device Controls