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The Zeiss ELYRA PS.1 is capable of both Structured Illumination Microscopy (SIM) and Photo-Activation Localisation Microscopy (PALM). In addition it can be configured for dSTORM, standard TIRF and wide-field microscopy applications. The microscope stage is enclosed in a blacked-out chamber for laser safety reasons and to maintain thermal stability. Super-resolution imaging is recommended and optimal at ambient temperature, but stage-top incubation equipment for live cell imaging applications is available. Multi-colour super-resolution imaging is also possible. The microscope is equipped with four laser lines 405, 488, 561 and 642 nm. The microscope is located in room G-09.
CONTENTS
QUICK START INSTRUCTIONS - Hardware & Software
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GETTING STARTED – PALM & 3D PALM Imaging & Image Processing
ZEISS ELYRA PS.1 TECHNICAL SPECIFICATIONS
LINKS
Background information on super-resolution techniques
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Interactive PALM tutorial: http://zeiss-campus.magnet.fsu.edu/tutorials/superresolution/palmbasics/indexflash.html
Quick start instructions poster
Sample preparation
Zeiss ELYRA Sample Preparation Quick Guide 2013.pdf
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Useful image analysis tools
SIMCheck to test the quality of your SIM images: http://www.micron.ox.ac.uk/software/SIMCheck.php
Super-Resolution Facility (SurF) access application form
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