Page Comparison

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OBJECTIVE LENSES
Objective	Magnification	Immersion Medium	Numerical Aperture	Coverslip correction [mm]	Working Distance [mm]
EC Plan-Neofluar	10x	air	0.3	0.17	5.2
LCI Plan-Neofluar Imm Korr DIC M27	25x	water	0.8	adjustable, 0 - 0.17	0.21 at cover glass 0.17
EC Plan-Neofluar OIL DIC M27	40x	oil	1.3	0.17	0.21
C-Apochromat Korr M27	63x	water	1.2	adjustable, 0.14 - 0.19	0.28 at cover glass 0.17
Plan-Apochromat DIC M27	63x	oil	1.4	0.17	0.19
alpha Plan-Apochromat DIC M27 Elyra	100x	oil	1.46	0.17	0.11

FLUORESCENCE FILTER SETS
Two 6-position emission filter turrets are available for imaging on the Zeiss ELYRA PS.1, one with single-bandpass filters and another one with multi-bandpass filters.

Single-band emission filter set
Turret position	Emission filter	Excitation filter	Example Fluorophores
1	Transmission light	N/A	N/A
2	Triple-band filter (labelled None)	N/A	most common fluorophores in the visible range
3	BP 420 - 480/LP 750	MBS 405	DAPI
4	BP 495 - 550/LP 750	MBS -488	GFP, Alexa Fluor 488
5	BP 470 - 620/LP 750	MBS -561	mCherry, Texas Red
6	LP 655	MBS -642	Cy5, Alexa Fluor 647

Multi-band emission filter set
Turret position	Emission filter	Excitation filter	Example Fluorophores
1	Empty	N/A	N/A
2	Empty	N/A	N/A
3	BP 420 - 480/BP 570 - 640/LP 740	MBS 405/561	DAPI & mCherry
4	BP 420 - 480/BP 495 - 550/LP 650	MBS 405/488/642	DAPI, GFP & Cy5
5	LBF -561/642	MBS -561/642	Alexa Fluor 555 & Alexa Fluor 647
6	LBF -488/561	MBS 405ac/488/561	DAPI, GFP & mCherry

CAMERA/DETECTOR
Camera	Pixels	Pixel Size [µm]	Quantum Efficiency (QE)
Andor iXon 897 (EMCCD) for PALM imaging (left side port)	512 x 512	16 x 16	90%
pco.egde sCMOS for SIM imaging (base port)	1280 x 1280	6.5 x 6.5	57%, dynamic range 15 bit

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