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- Follow the QUICK START INSTRUCTIONS and the guide on GETTING STARTED - PUTTING A SPECIMEN ON AND LOCATING IT USING THE OCULAR for the N-STORM microscope. Make sure to select the Andor Zyla sCMOS camera during the software start-up procedure.
- At the bottom of the NIS-Elements software window select the Basic Widefield tab to go to the widefield acquisition layout.
- The lightpath for epifluorescence imaging should already be configured from the previous step in which you used the eyepiece of the microscope to focus on your sample. Therefore you should only have to click the L100 icon under Light Path in the TiPad to direct the light to the sCMOS camera. Also check that the LED (1) you would like to use to excite fluorescence of your sample is turned on and move the power slider to the desired value. Make sure that the Spectra shutter (2) is open the TIRF/EPI switch (3) is in the EPI position and the correct emission filter (4) for your fluorophore of interest is selected (blue, e.g. DAPI; green, e.g. GFP or Alexa Fluor 488; red, e.g. mCherry or Texas Red; SpectraX Quad for fast imaging of multiple colours with the same filter).