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Mount your specimen on the stage. Direct light to the microscope eyepieces by pressing the E100 button on the front operation panel. Press the FL1 button on the Ti-RCP remote control panel and select the empty filter position (-----). Turn on the pE100 and set the brightness to a suitable level. Coarsely adjust the focus of the condenser so the two pieces of orange tape line up. If you are using the CO2 chamber be careful not to break the lid with the condenser lens. Use a low power lens like the 10x to focus on your specimen and then adjust the condenser for Köhler illumination. Once the microscope is in Köhler rotate the correct phase ring into position. Check the list of objective lenses to see which phase ring to use.
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Aligning the microscope for Köhler illuminationFully close the field diaphragm and adjust the condenser height so the image of the iris is in sharp focus. Centre the image of the iris using the two centring screws. Open the field diaphragm until it is just outside the field of view. Remove an eyepiece and look into the empty opening. Adjust the iris aperture until it is about 70% open and then replace the eyepiece. The microscope is now adjusted for Köhler illumination. |
View fluorescence down the eyepieces
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