GFP3 Objectives
- Andrew Vaughan
Owned by Andrew Vaughan
| Objective Lenses | |||||
|---|---|---|---|---|---|
| Magnification | Immersion | Type | Phase Contrast | NA | WD (mm) |
| 4x | Air | Plan Fluor | - | 0.13 | 17.1 |
| 10x | Air | Plan Fluor | Ph1 | 0.3 | 15.2 |
| 20x | Air | Plan Fluor ELWD | Ph1 | 0.45 | 7.4 |
| 40x | Air | Plan Fluor ELWD | Ph2 | 0.6 | 3.7 |
Lenses are corrected for #1.5 coverslips (0.17 micron thick) except ELWD (extra long working distance) NA = Numerical Aperture, WD = Working Distance | |||||
We also have the following lenses for use with #1.5 coverslips:
- 20x Plan Fluor NA 0.5 Ph1 air objective lens with WD 2.1 (not compatible with Perfect Focus System)
- 40x Plan Fluor NA 0.75 Ph2 air objective lens with WD 0.66
- 60x Plan Apo λ NA 1.4 Ph3 oil lens with WD 0.13.
There is a choice of two immersion oils:
Oil | Refractive Index | For use with... | Kept in... |
|---|---|---|---|
Cargille Type HF | 1.518 (at 23ºC) | Oil lenses at room temperature | Tray outside environmental chamber |
Cargille Type 37 | 1.518 (at 37°C) | Oil lenses at 37ºC | Tray inside environmental chamber |
Note that Cargille Type 37 is optimal for use at 37ºC but introduces spherical aberrations when imaging beyond about 10 μm in aqueous media because it is not refractive indexed matched to water.