GFP3 Objectives

Objective Lenses
MagnificationImmersionTypePhase ContrastNAWD (mm)
4xAirPlan Fluor-0.1317.1
10xAirPlan FluorPh10.315.2
20xAirPlan Fluor ELWDPh10.457.4
40xAirPlan Fluor ELWDPh20.63.7

Lenses are corrected for #1.5 coverslips (0.17 micron thick) except ELWD (extra long working distance)

NA = Numerical Aperture, WD = Working Distance

We also have the following lenses for use with #1.5 coverslips:

  • 20x Plan Fluor NA 0.5 Ph1 air objective lens with WD 2.1 (not compatible with Perfect Focus System)
  • 40x Plan Fluor NA 0.75 Ph2 air objective lens with WD 0.66
  • 60x Plan Apo λ NA 1.4 Ph3 oil lens with WD 0.13.

There is a choice of two immersion oils:

Oil

Refractive Index

For use with...

Kept in...

Cargille Type HF

1.518 (at 23ºC)

Oil lenses at room temperature

Tray outside environmental chamber

Cargille Type 37

1.518 (at 37°C)

Oil lenses at 37ºC

Tray inside environmental chamber

Note that Cargille Type 37 is optimal for use at 37ºC but introduces spherical aberrations when imaging beyond about 10 μm in aqueous media because it is not refractive indexed matched to water.