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Mount your specimen on the stage. Direct light to the microscope eyepieces by pressing the EYE button on the front operation panel. Press the FL1 button on the Ti-RCP remote control panel and select the empty filter position (-----). Turn on the pE100 and set the brightness to a suitable level. Coarsely adjust the focus of the condenser so the two pieces of orange tape line up (see red arrow below). If you are using the CO2 chamber be careful not to break the lid with the condenser lens. Use a low power lens like the 10x to focus on your specimen and then adjust the condenser for Köhler illumination. Once the microscope is in Köhler rotate the correct phase ring into position. Check the list of objective lenses to see which phase ring to use.
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