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- Turn on the "Main switch" on the keypad
- Turn on "Systems/PC" on the keypad
- Wait for computer to boot up and log in to LSM-User account
- Turn on the "Components" switch on the keypad.
- Click the "Start System" button
Click the" Acquisition" tab and expand the "Laser" menu. Turn on the lasers you need. (Some of the lasers might show a red background initially, which means it is not ready to be used. This will go away after a couple of mintues when the laser is warmed up)
Locating your Specimen
- Click the "Locate" tab and expand the "Microscope Control" drop-down menu
- Select the objective lens you'd like to use - either via the menu in locate', or via the touchscreen controller next to the screen.
- Select a suitable fluorescence filter. Only the first three should be used; the rest are special positions fof the confocal and multiphoton
- Put your specimen on the microscope and open either the transmitted light shutter or the fluorescence shutter depending on which mode you want to use to find your sample
- Turn on the lamp by clicking its icon and pressing the On button
- Open the fluorescence shutter to let light through
- When your specimen is in focus, close the shutter and click the "Acquisition" tab.
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