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On the panel below the computer monitor, turn on Microscope, Confocal, Lasers and Lamp.  Turn on Photomanipulation if you intend to do a photokinetics experiment (FRAP, FRET, FLIM etc)

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If you need CO2, the manual switches are behind the computer.  You'll have to go round the back and manually toggle the flow switches into the off or on positions.  

The wall mixer dispenses both 5% CO2 and 10% CO2.  If the flow controller is perpendicular to the tubing, the flow is off and if it is in-line with the tubing, the flow is on.  You can check that the flow is really on by checking if the indicator is floating in the flow regulator.

There is a laser safety key at the top of the laser tower.  To initialise the safety shutter, turn the key to off and back to on again if the key had been left in the off position after the system's been shut down previously.

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When the computer starts up, Windows will usually automatically log you into the last user account.  At the moment this is standard practice, however we may introduce individual user accounts sometime in the future.  Open SlideBook 6 software to begin using the microscope.  

To shut down : first shut down windows on the computer.  Next turn off all the switches.