On the panel below the computer monitor, turn on the buttons for Photomanipulation, Lamp, Microscope, Confocal , and Lasers and Lamp. Turn on Photomanipulation if you intend to do a photokinetics experiment (FRAP, FRET, FLIM etc)
Then, switch on the computer at the bottom of the 3I tower
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If you need CO2, the manual switches are behind the computer. You'll have to go round the back and manually toggle the flow switches into the off or on positions.
The wall mixer dispenses both 5% CO2 and 10% CO2. If the flow controller is perpendicular to the tubing, the flow is off and if it is in-line with the tubing, the flow is on. You can check that the flow is really on by checking if the indicator is floating in the flow regulator.
There is a laser safety You must turn off the key at the top of the laser tower . To initialise the safety shutter, turn the key to off and back to on again if the key had been left in the off position after the system's been shut down previously.
and immediately turn it back on again to open the laser safety shutter. The lasers will be blocked if you don't do this. If you are trying to image but no laser light is coming out of the objective lens, you may have forgotten to do this.
When the computer starts up, Windows will usually automatically log you into the last user account. At the moment this is standard practice, however we may introduce individual user accounts sometime in the future. Open SlideBook 6 software to begin using the microscope.
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