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If you wish to use chambered coverslips, we recommend the 2 or 4 chambered ones. the 8-chambered ones are impossible to image with the high NA objectives as the objective has to get too close to the 'arms' of the stage insert as well. This causes huge amounts of frustration to users, and potential damage to the objectives. You will not be able to image the whole surface area of your chambers. The middle 'aisle' of the slide is about the best that we can manage with this sample holder. We also cannot guarantee for TIRF to TIRF may not work with chambered coverslips.