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| Magnification | Immersion | Type | DIC | NA | Cover slip correction | WD (mm) |
|---|---|---|---|---|---|---|
| 10x | Air | Plan Apo λ | DIC L | 0.45 | 0.17 | 4 |
| 20x | Air | Plan Apo | DIC M | 0.75 | 0.17 | 1 |
| 40x | Air | Plan Apo | DIC M | 0.95 | 0.11 - 0.23 | 0.21 |
| 60xA | Oil | Plan Apo | DIC N2 | 1.4 | 0.17 | 0.21 |
| 100x | Oil | Plan Apo λ | - | 1.45 | 0.17 | 0.13 |
| 100x† | Silicone Oil | SR HP Plan Apo λS | - | 1.35 | 0.15 - 0.19 | 0.3 |
†The 100x silicone oil lens is available on request. NA = Numerical Aperture, WD = Working Distance | ||||||
Fluorescence filter sets
Wide-field Fluorescence Filters
Sedat quad-band dichroic and single-band emission filters in the filter wheel on the right-hand port. Lower speed but reduced cross-talk between channels.
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Filter ID | Excitation Wavelength | Dichroic Mirror | Emission Filter (in quad) | Example Fluorophores |
|---|---|---|---|---|
| DAPI | 385 nm | 86012v2 Dapi/FITC/TxRed/Cy5 | ~ 455nm - 485nm | DAPI, Hoechst |
| FITC | 470 nm | 86012v2 Dapi/FITC/TxRed/Cy5 | ~ 510nm - 545nm | GFP, FITC |
| TxRed | 550 nm | 86012v2 Dapi/FITC/TxRed/Cy5 | ~ 590nm - 630nm | RFP, mCherry |
| Cy5 | 635 nm | 86012v2 Dapi/FITC/TxRed/Cy5 | ~ 670nm - 740nm | Cy5 |
iSIM Filters
The iSIM has the following quad-band emission filter in its scan head:
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