The 3i Spinning Disk Confocal is equipped with a variety of stage inserts to fit a myriad of sample vessels. The insert fitted onto the stage when you start up would be what the previous user was using, and you may find that you'll have to change it. The inserts are usually located to the left of the microscope, inside the chamber. 
There are two inserts, both with ASI Imaging written on them, which fit directly onto the stage. These are a multi-well plate holder, and a universal K-type holder. Neither of this are connected to the CO2 supply. 
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If you wish to do multiwell imaging without CO2, you can use the CO2 chamber without any insertswith the modified MultiWell plate insert.
