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The objective lenses below have a larger M32 thread. To install these lenses the standard nosepiece must be removed from the microscope and replaced with a single-position nosepiece. Hence only one of these lenses can be installed at any one time. They have motorised correction collars which can be adjusted from within the software to correct for refractive index mismatches.
| Magnification | Type | NA | Immersion | WD | Part Number |
|---|---|---|---|---|---|
| 25x | HC IRAPO L motCORR | 1.0 | Water dipping | 2.6 | 507704 |
| 25x | HC FLUOTAR L motCORR VISIR | 1.0 | Clarity | 6 | 507703 |
Note that the 6 mm working distance of the Clarity lens will mean that any sample imaged using this lens will most likely have to be mounted in a deep vessel.
Lasers
The DIVE has the following visible solid state lasers for confocal microscopy.
| Wavelength | Colour | Example dyes |
|---|---|---|
| 405 | DAPI, Hoechst | |
| 448 | CFP | |
| 488 | GFP, Alexa 488 | |
| 514 | YFP | |
| 561 | mCherry, Alexa 568 | |
| 638 | Cy5, Alexa 647 |